Schmid Ingrid, Lambert Claude, Ambrozak David, Perfetto Stephen P
David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
Curr Protoc Cytom. 2007 Jan;Chapter 3:Unit3.6. doi: 10.1002/0471142956.cy0306s39.
Cell sorting of viable biological specimens has become widespread in laboratories involved in basic and clinical research. As these samples can contain infectious agents, precautions to protect instrument operators and the environment from hazards arising from the use of sorters are paramount. This unit presents a revised and updated version of the biosafety guidelines for sorting of unfixed cells established in 1977 by the International Society of Analytical Cytology (ISAC), whose recommendations have become recognized worldwide as the standard practices and safety precautions for laboratories performing viable cell-sorting experiments. The unit contains background information on the biohazard potential of sorting and the hazard classification of infectious agents as well as recommendations on (1) sample handling, (2) operator training and personal protection, (3) laboratory design, (4) cell sorter setup, maintenance, and decontamination, and (5) testing the instrument for the efficiency of aerosol containment.
活生物样本的细胞分选在基础研究和临床研究实验室中已广泛应用。由于这些样本可能含有感染性因子，采取预防措施以保护仪器操作人员和环境免受分选仪使用所产生危害至关重要。本单元介绍了国际分析细胞学协会（ISAC）于1977年制定的未固定细胞分选生物安全指南的修订和更新版本，其建议已在全球范围内被认可为进行活细胞分选实验的实验室的标准操作和安全预防措施。本单元包含关于分选的生物危害潜力和感染性因子危害分类的背景信息，以及关于（1）样本处理、（2）操作人员培训和个人防护、（3）实验室设计、（4）细胞分选仪设置、维护和去污，以及（5）检测仪器气溶胶 containment 效率的建议。
