Holmes Kevin L, Fontes Benjamin, Hogarth Philip, Konz Richard, Monard Simon, Pletcher Charles H, Wadley Robert B, Schmid Ingrid, Perfetto Stephen P
Flow Cytometry Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Cytometry A. 2014 May;85(5):434-53. doi: 10.1002/cyto.a.22454. Epub 2014 Mar 13.
Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument operators and the environment from biohazards arising from the use of sorters. To this end the International Society of Analytical Cytology (ISAC) was proactive in establishing biosafety guidelines in 1997 (Schmid et al., Cytometry 1997;28:99-117) and subsequently published revised biosafety standards for cell sorting of unfixed samples in 2007 (Schmid et al., Cytometry Part A J Int Soc Anal Cytol 2007;71A:414-437). Since their publication, these documents have become recognized worldwide as the standard of practice and safety precautions for laboratories performing cell sorting experiments. However, the field of cytometry has progressed since 2007, and the document requires an update. The new Standards provides guidance: (1) for laboratory design for cell sorter laboratories; (2) for the creation of laboratory or instrument specific Standard Operating Procedures (SOP); and (3) on procedures for the safe operation of cell sorters, including personal protective equipment (PPE) and validation of aerosol containment.
生物样本的流式细胞术细胞分选在基础和临床研究实验室中已变得很普遍。这些样本可能含有已知或未知的传染源，因此需要采取预防措施，以保护仪器操作人员和环境免受因使用分选仪而产生的生物危害。为此，国际分析细胞学协会（ISAC）于1997年积极制定了生物安全指南（施密德等人，《细胞计数》1997年；第28卷：99 - 117页），随后在2007年发布了针对未固定样本细胞分选的修订生物安全标准（施密德等人，《细胞计数A部分：国际分析细胞学杂志》2007年；第71A卷：414 - 437页）。自发布以来，这些文件已被全球公认为进行细胞分选实验的实验室的实践和安全预防标准。然而，自2007年以来，细胞计数领域取得了进展，该文件需要更新。新的标准提供了以下指导：（1）关于细胞分选仪实验室的实验室设计；（2）关于制定实验室或仪器特定的标准操作规程（SOP）；以及（3）关于细胞分选仪安全操作的程序，包括个人防护装备（PPE）和气溶胶遏制验证。
