Zoology Department, Alexandria University, Alexandria, Egypt. dr.heba
J Environ Sci Health B. 2012;47(4):306-14. doi: 10.1080/03601234.2012.640913.
The involvement of reactive oxygen species (ROS) has been implicated in the toxicity of various pesticides. Our study was designed to investigate the induction of oxidative stress by cypermethrin; a Type II pyrethroid in rat liver and kidney. In addition, the protective role of sesame oil against the toxicity of cypermethrin was investigated. Animals were divided into four equal groups; the first group used as control while groups 2, 3 and 4 were treated with sesame oil (5 mL/kg b.w), cypermethrin (12 mg/kg b.w) and the combination of both sesame oil (5 mL/kg b.w) plus cypermethrin (12 mg/kg b.w), respectively. Rats were daily administered with their respective doses for 30 days by gavage. Repeated oral administration of cypermethrin was found to reduce the level of glutathione (GSH) and the activities of the antioxidant enzymes. While, the level of TBARS was elevated indicating the presence of oxidative stress. The activities of LDH, AST and ALT were decreased in the liver extract while increased in the plasma of the cypermethrin-treated group. Also, the levels of urea and creatinine were significantly increased after treatment with cypermethrin. Liver and kidney injury was confirmed by the histological changes. In conclusion, the administration of sesame oil provided significant protection against cypermethrin-induced oxidative stress, biochemical changes, histopathological damage and genomic DNA fragmentation.
活性氧（ROS）的参与与各种农药的毒性有关。我们的研究旨在研究氯菊酯（一种 II 型拟除虫菊酯）在大鼠肝和肾中诱导氧化应激的情况。此外，还研究了芝麻油对氯菊酯毒性的保护作用。动物被分为四组，第一组作为对照组，第二、三、四组分别用芝麻油（5 毫升/公斤体重）、氯菊酯（12 毫克/公斤体重）和芝麻油（5 毫升/公斤体重）加氯菊酯（12 毫克/公斤体重）处理。大鼠通过灌胃每天给予各自的剂量 30 天。重复口服氯菊酯被发现降低了谷胱甘肽（GSH）的水平和抗氧化酶的活性。而 TBARS 的水平升高表明存在氧化应激。肝提取物中 LDH、AST 和 ALT 的活性降低，而氯菊酯处理组的血浆中则升高。此外，用氯菊酯处理后，尿素和肌酐的水平显著升高。肝和肾损伤通过组织学变化得到证实。总之，芝麻油的给药对氯菊酯诱导的氧化应激、生化变化、组织病理学损伤和基因组 DNA 片段化提供了显著的保护作用。
