Laboratorio de Biología Celular y Retrovirus-CONICET, Hospital de Pediatría J.P. Garrahan, Buenos Aires, Argentina.
Infect Genet Evol. 2011 Mar;11(2):349-57. doi: 10.1016/j.meegid.2010.11.008. Epub 2010 Dec 3.
The HIV-1 epidemic associated to BF1 recombinants in South America is both complex and intriguing, with an underestimated diversity of recombinant structures. Our aim was to explore the characteristics and temporal dynamics of the HIV-1 BF1 epidemic in Argentina, through the study of 172 HIV-1 pol BF1 recombinant sequences obtained from HIV-1 vertically infected patients born from 1986 to 2008. Recombination patterns were characterized by bootscanning, subtype signature analysis, and phylogenetic approaches. Proportion of sequences sharing common ancestry and recombination breakpoints with the Circulating Recombinant Form (CRF) CRF12_BF was compared against sequences with a non-CRF12_BF pattern in three study periods, and by fitting the data to a logistic model. Twenty-eight HIV-1 pol BF1 mosaic structures were identified, including four of the seven South-American CRF_BF-like patterns. However, common ancestry of these sequences with reference CRF strains only confirmed the presence of CRF12_BF (51.1%) and CRF17_BF (1.2%) among the Argentine BF pol sequences. Most non-CRF_BF-like recombinant patterns shared at least one common recombination breakpoint with CRF12_BF. The number of transmissions caused by CRF12_BF viruses decreased in a linear way over time, from 69% in the period 1986-1993 to 46% in 2001-2008. In conclusion, the diversity of HIV-1 pol BF1 recombinant structures in Argentina is much more complex than previously described, with at least two CRFs_BF and 26 BF1 unique recombinant forms. For the first time, we provide evidence of a decrease in the proportion of CRF12_BF viruses transmitted from mother-to-child since the start of the epidemic to the present time in Argentina.
在南美洲，与 BF1 重组体相关的 HIV-1 流行情况既复杂又有趣，重组体结构的多样性被低估。我们的目的是通过研究 1986 年至 2008 年间出生的 HIV-1 垂直感染患者的 172 个 HIV-1 pol BF1 重组序列，探索阿根廷 HIV-1 BF1 流行的特征和时间动态。通过 bootscanning、亚型特征分析和系统发育方法来描述重组模式。通过在三个研究时期将与 Circulating Recombinant Form (CRF) CRF12_BF 共享共同祖先和重组断点的序列比例与具有非-CRF12_BF 模式的序列进行比较，并将数据拟合到逻辑模型中，来比较序列。确定了 28 个 HIV-1 pol BF1 镶嵌结构，包括 7 个南美 CRF_BF 样模式中的 4 个。然而，这些序列与参考 CRF 株的共同祖先仅证实了阿根廷 BF pol 序列中存在 CRF12_BF（51.1%）和 CRF17_BF（1.2%）。大多数非-CRF_BF 样重组模式与 CRF12_BF 至少共享一个共同的重组断点。CRF12_BF 病毒引起的传播数量随着时间的推移呈线性下降，从 1986 年至 1993 年的 69%下降到 2001 年至 2008 年的 46%。总之，阿根廷 HIV-1 pol BF1 重组结构的多样性比以前描述的要复杂得多，至少有两个 CRFs_BF 和 26 个 BF1 独特的重组形式。我们首次提供了证据，表明自阿根廷流行开始至今，母婴传播的 CRF12_BF 病毒比例有所下降。
