Center for Infectious and Inflammatory Diseases at Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, Texas 77030, USA.
J Biol Chem. 2011 Feb 11;286(6):4517-24. doi: 10.1074/jbc.M110.159590. Epub 2010 Dec 3.
Toll-like receptors (TLRs) are the key molecular sensors used by the mammalian innate immune system to detect various types of pathogens. Tlr13 is a novel and uncharacterized member of the mammalian TLR family. Here we report the cloning and characterization of tlr13. Tlr13 is predominantly expressed in the spleen, particularly in dendritic cells and macrophages. Tlr13 appears to activate a MyD88- and TAK1-dependent TLR signaling pathway, inducing the activation of NF-κB. This receptor can also activate type 1 interferon through IRF7. Furthermore, Tlr13 seems to be another intracellular TLR. Remarkably, cells expressing tlr13 fail to respond to known TLR ligands but instead respond specifically to vesicular stomatitis virus. Cells with the knockdown of tlr13 are highly susceptible to vesicular stomatitis virus infection. Thus, these results provide an important insight into the potential role of the novel Toll-like receptor tlr13 in the recognition of viral infection.
Toll-like 受体 (TLRs) 是哺乳动物先天免疫系统用于检测各种病原体的关键分子传感器。Tlr13 是哺乳动物 TLR 家族中的一个新的未被描述的成员。在这里，我们报道了 tlr13 的克隆和特性。Tlr13 主要在脾脏中表达，特别是在树突状细胞和巨噬细胞中。Tlr13 似乎激活了一个依赖于 MyD88 和 TAK1 的 TLR 信号通路，诱导 NF-κB 的激活。这种受体还可以通过 IRF7 激活 I 型干扰素。此外，Tlr13 似乎是另一种细胞内 TLR。值得注意的是，表达 tlr13 的细胞对已知的 TLR 配体没有反应，但对水疱性口炎病毒有特异性反应。tlr13 敲低的细胞对水疱性口炎病毒感染高度敏感。因此，这些结果为新型 Toll-like 受体 tlr13 在识别病毒感染中的潜在作用提供了重要的见解。
