凝集素微阵列。
Lectin microarray.
机构信息
UCLA School of Dentistry and Dental Research Institute, Los Angeles, CA, USA.
UCLA Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA.
出版信息
Proteomics Clin Appl. 2009 Feb;3(2):148-54. doi: 10.1002/prca.200800153.
Abstract
Lectin microarray is a new technology that utilizes a panel of lectins immobilized on well-defined substrate for high-throughout analysis of glycans and glycoproteins. In this article, we have reviewed the fabrication and detection schemes in lectin microarray and discussed its novel applications in glycomics. We have also demonstrated a lectin array on PDMS with MALDI-TOF-MS for glycoprotein analysis. This method has been demonstrated for differential analysis of serum glycoproteins in oral cancer and healthy control subjects.
摘要
凝集素微阵列是一种新技术，它利用固定在明确基质上的凝集素阵列进行高通量糖和糖蛋白分析。在本文中，我们综述了凝集素微阵列的制作和检测方案，并讨论了其在糖组学中的新应用。我们还展示了一种基于 PDMS 的凝集素阵列与 MALDI-TOF-MS 联用用于糖蛋白分析的方法。该方法已用于口腔癌患者和健康对照者血清糖蛋白的差异分析。
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