Leuchtweis J, Imhof A-K, Montechiaro F, Schaible H-G, Boettger M K
Institute of Physiology I/Neurophysiology, University Hospital, Jena, Germany.
Methods Find Exp Clin Pharmacol. 2010 Oct;32(8):575-83. doi: 10.1358/mf.2010.32.8.1532102.
Several tests have been developed to obtain mechanical nociceptive withdrawal thresholds for arthritis-associated pain research in preclinical animal models, which are routinely used for testing the efficacy of antinociceptive pharmaceutical candidates. Here, we aimed to validate a recently introduced and commercially available digital pressure application measurement (PAM) device for the detection of primary mechanical hyperalgesia in a model of antigen-induced knee joint arthritis (AIA) in rats and mice. Two particular advantages of the PAM device are visual feedback control of the force increase rate and the detection of the complete threshold range. Using PAM, we were able to quantify mechanical thresholds at the knee joint in rats and mice (400 and 350 g, respectively) before and during the time course of AIA (approximately 100 g for rats and mice in the acute phase). Inter-observer agreement was generally higher when using PAM instead of an analog dynamometer. In conclusion, the digital PAM device is a suitable apparatus to detect primary mechanical hyperalgesia in experimental knee joint arthritis in rats and mice. The use of this device allows visual feedback control of the stimulus rate, thus minimizing the chances of confounding factors arising from differences in ramp speed.
已经开发了几种测试方法来获得临床前动物模型中与关节炎相关疼痛研究的机械性伤害感受性退缩阈值，这些方法通常用于测试抗伤害性药物候选物的疗效。在此，我们旨在验证一种最近推出的市售数字压力应用测量（PAM）设备，用于检测大鼠和小鼠抗原诱导性膝关节关节炎（AIA）模型中的原发性机械性痛觉过敏。PAM设备的两个特别优点是力增加速率的视觉反馈控制和完整阈值范围的检测。使用PAM，我们能够在AIA病程之前和期间量化大鼠和小鼠膝关节的机械阈值（分别为400和350克）（急性期大鼠和小鼠约为100克）。使用PAM时，观察者间的一致性通常高于使用模拟测力计。总之，数字PAM设备是检测大鼠和小鼠实验性膝关节关节炎中原发性机械性痛觉过敏的合适仪器。使用该设备可以对刺激速率进行视觉反馈控制，从而最大限度地减少因斜坡速度差异而产生混杂因素的可能性。
