亚硝基胍诱导的铜绿假单胞菌适应性修复

Nitrosoguanidine-induced adaptive repair in Pseudomonas aeruginosa.

作者信息

Rasool S A, Mirza A, Khan M A

机构信息

Department of Microbiology, University of Karachi, Pakistan.

出版信息

Curr Genet. 1990 May;17(5):417-9. doi: 10.1007/BF00334521.

DOI:10.1007/BF00334521

PMID:2113435

Abstract

Error-proof adaptive repair has been demonstrated in Pseudomonas aeruginosa. Cells of actively replicating wild-type Ps. aeruginosa (ATCC27853) and its auxotrophic derivative PAO 286 were subjected to stepwise adaptation (up to 1 microgram ml-1) by nitrosoguanidine (MNNG). Such cells resisted lethal and mutagenic effects of MNNG-challenge (lethal) doses more efficiently than those of nonadapted cultures. Similarly, reactivation of alkylated Pseudomonas phages was enhanced in adapted cells only. Induction of adaptive repair enzymes was sensitive to chloramphenicol (protein synthesis-inhibiting antibiotic) during adaptation treatment only. Protein extract from adapted cells showed increased levels in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).

摘要

抗错适应性修复已在铜绿假单胞菌中得到证实。对活跃复制的野生型铜绿假单胞菌（ATCC27853）及其营养缺陷型衍生物PAO 286的细胞，用亚硝基胍（MNNG）进行逐步适应性处理（高达1微克/毫升）。与未适应培养的细胞相比，这类细胞能更有效地抵抗MNNG挑战（致死）剂量的致死和诱变作用。同样，仅在适应细胞中烷基化假单胞菌噬菌体的重新激活得到增强。仅在适应处理期间，适应性修复酶的诱导对氯霉素（蛋白质合成抑制抗生素）敏感。适应细胞的蛋白质提取物在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中显示水平升高。