益肾蠲痹丸（YJB）对实验性关节炎的体内外作用。
Ex vivo and in vivo effect of Chinese herbal pill Yi Shen Juan Bi (YJB) on experimental arthritis.
机构信息
Department of Physiology, China Pharmaceutical University, Mailbox 207 Tongjiaxiang 24, Nanjing, Jiangsu, 210009, PR China.
出版信息
J Ethnopharmacol. 2011 Mar 8;134(1):171-5. doi: 10.1016/j.jep.2010.11.065. Epub 2010 Dec 4.
AIM OF THIS STUDY
Chinese herbal pill Yi Shen Juan Bi (YJB), which is a compound herbal drug, has traditionally been used as an anti-rheumatic drug in Chinese local clinics. Thus, we investigated the therapeutic effect of the YJB in rheumatoid arthritis (RA), using Freund's complete induced adjuvant arthritis (AA) in rat model.
METHODS
For that purpose, macrophage derived cytokine tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1) were measured in ex vivo by enzyme linked immunosorbent assay (ELISA). We also assayed the effect of YJB on peritoneal macrophage derived nitric oxide (NO) in ex vivo by Griess reaction. Prostaglandin E (PGE) in metapedes was assayed by ultraviolet spectrophotometer method. Further synovial Bax level was examined by Western blot analysis.
RESULTS
YJB significantly decreased the production of peritoneal macrophages derived TNF-α, IL-1 and NO. YJB also significantly decreased prostaglandin E (PGE) and upregulated the Bax expression in AA rat's synovium.
CONCLUSION
YJB is a potential anti-rheumatic agent targeting the inflammatory and immunomodulatory response of macrophages while down regulating the PGE and up-regulating the pro-apoptotic Bax expression. Such characteristics of YJB on AA may be advantageous to the treatment of clinical rheumatoid arthritis.
本研究目的
益肾蠲痹丸（YJB）是一种复方草药，传统上被用作中国当地诊所的抗风湿药物。因此，我们使用完全弗氏佐剂诱导的关节炎（AA）大鼠模型，研究 YJB 在类风湿关节炎（RA）中的治疗效果。
方法
为此，通过酶联免疫吸附试验（ELISA）在体外测量巨噬细胞衍生的细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1（IL-1）。我们还通过格里斯反应测定 YJB 对体外腹腔巨噬细胞衍生的一氧化氮（NO）的影响。通过紫外分光光度计法测定甲壳质中的前列腺素 E（PGE）。通过 Western blot 分析进一步检查滑膜 Bax 水平。
结果
YJB 显著降低了腹腔巨噬细胞衍生的 TNF-α、IL-1 和 NO 的产生。YJB 还显著降低了 AA 大鼠滑膜中的前列腺素 E（PGE），并上调了 Bax 的表达。
结论
YJB 是一种潜在的抗风湿药物，针对巨噬细胞的炎症和免疫调节反应，同时下调 PGE 并上调促凋亡 Bax 的表达。YJB 对 AA 的这些特征可能有利于治疗临床类风湿关节炎。
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