Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Frei Caneca 94, 20.261-040, Rio de Janeiro, Rio de Janeiro, Brazil.
J Clin Immunol. 2011 Apr;31(2):186-94. doi: 10.1007/s10875-010-9485-3. Epub 2010 Dec 7.
Our objective was to evaluate the in vitro functional profile of T cells from uninfected neonates born from HIV-1-infected pregnant women who controlled (G1) or not (G2) the virus replication. We demonstrated that the lymphoproliferation of T cell to polyclonal activators was higher in the G2 as compared with G1. Nevertheless, no detectable proliferative response was observed in response to HIV-1 antigens in both neonate groups. Cytokine dosage in the supernatants of these polyclonally activated T cell cultures demonstrated that, while IL-10 was the dominant cytokine produced in G1, Th17-related cytokines were significantly higher in G2 neonates. The higher Th17 phenotype tendency in G2 was related to high production of IL-23 by lipopolysaccharide-activated monocyte-derived dendritic cells from these neonates. Our results demonstrated immunological disorders in uninfected neonates born from viremic HIV-1-infected mothers that can help to explain why some of these children have elevated risk of clinical morbidity and mortality due to pathological hypersensitivity.
我们的目的是评估来自 HIV-1 感染孕妇的未感染新生儿的 T 细胞的体外功能特征，这些孕妇能够（G1 组）或不能（G2 组）控制病毒复制。我们证明，与 G1 组相比，G2 组 T 细胞对多克隆激活剂的淋巴增殖更高。然而，在这两个新生儿组中，均未观察到针对 HIV-1 抗原的可检测增殖反应。这些多克隆激活的 T 细胞培养物上清液中的细胞因子剂量表明，虽然 G1 组中产生的主要细胞因子是 IL-10，但 G2 组新生儿中与 Th17 相关的细胞因子显著更高。G2 中更高的 Th17 表型倾向与这些新生儿的脂多糖激活单核细胞衍生树突状细胞中 IL-23 的高产量有关。我们的研究结果表明，来自病毒血症 HIV-1 感染母亲的未感染新生儿存在免疫紊乱，这可以帮助解释为什么这些儿童中的一些有更高的临床发病和死亡风险，这是由于病理性过敏。
