Zhongnan hospital, Wuhan University, Wuhan, China.
Lett Appl Microbiol. 2011 Jan;52(1):76-83. doi: 10.1111/j.1472-765X.2010.02969.x. Epub 2010 Dec 8.
To develop a new nano-composite of multi-walled carbon nanotubes (MWNTs) with enhanced antimicrobial activity.
A novel antimicrobial nanocomposite [MWNT-epilson-polylysine (MEPs)] was synthesized via covalent attachment of epilson-polylysine on MWNTs with hexamethylene diisocyanate (HDI) as the coupling agent. UV-visible spectra and Fourier transform infrared spectra (FT-IR) investigations indicate that MEPs is stable, with epilson-polylysine leaching effectively eliminated. When compared to MWNTs, the new nano-composite MEPs exhibits enhanced antimicrobial activities. In 20 mg l(-1) suspensions, significant increases of 72·1, 64·5 and 69% against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus can be observed. The deposited film of MEPs also shows improved antibacterial activities and excellent antiadhensive efficacies against Ps. aeruginosa and Staph. aureus.
Epilson-polylysine functionalization of MWNTs with HDI as the bridge was found to be useful for improving the biocidal activity of MWNTs.
The new nano-composite MEPs with improved antimicrobial activity will substantially facilitate the application of MWNTs as the antimicrobial material such as medical device, food, pharmaceutical process and package.
开发一种具有增强抗菌活性的新型多壁碳纳米管（MWNTs）纳米复合材料。
通过将ε-聚赖氨酸共价连接到 MWNTs 上，并用己二异氰酸酯（HDI）作为偶联剂，合成了一种新型抗菌纳米复合材料[MWNT-ε-聚赖氨酸（MEPs）]。紫外可见光谱和傅里叶变换红外光谱（FT-IR）研究表明，MEPs 是稳定的，ε-聚赖氨酸的浸出得到了有效消除。与 MWNTs 相比，新型纳米复合材料 MEPs 表现出增强的抗菌活性。在 20mg/L 的悬浮液中，对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抗菌活性分别显著提高了 72.1%、64.5%和 69%。MEPs 的沉积膜也显示出改善的抗菌活性和对铜绿假单胞菌和金黄色葡萄球菌的优异抗黏附功效。
用 HDI 作为桥接物对 MWNTs 进行ε-聚赖氨酸功能化，有助于提高 MWNTs 的杀菌活性。
具有增强抗菌活性的新型纳米复合材料 MEPs 将极大地促进 MWNTs 作为抗菌材料（如医疗器械、食品、制药工艺和包装）的应用。
