山羊能否用作非流行地区蜱传脑炎（TBE）的哨兵？实验研究和动物流行病学观察。

Can goats be used as sentinels for tick-borne encephalitis (TBE) in nonendemic areas? Experimental studies and epizootiological observations.

作者信息

Klaus Christine, Hoffmann Bernd, Moog Udo, Schau Ute, Beer Martin, Süss Jochen

机构信息

Friedrich-Loeffler-Lnstitute, Jena, Germany.

出版信息

Berl Munch Tierarztl Wochenschr. 2010 Nov-Dec;123(11-12):441-5.

PMID:21141272

Abstract

A goat flock grazing year-round on a meadow in a "TBE non-risk area" in Thuringia, Germany, with a history of only isolated human TBE cases was examined repeatedly for TBE virus-(TBEV)-specific antibodies and TBEV RNA between October 2008 and December 2009. Surprisingly, TBEV specific antibodies were detected in one goat, which had never left this area. To compare the results of a natural contact to TBEV with a defined contact to TBEV, two goats were immunized experimentally. Both animals developed TBEV-specific antibodies, one goat however in a delayed and reduced manner. In addition, 177 ticks were collected from the meadow in May and June 2009, and were examined by real-time RT-PCR. However, noTBEV RNA could be detected. The results suggest that goats can be used as TBEV sentinels in defined areas. To verify this observation further investigations with a large number of animals are recommended.

摘要

德国图林根州一个“蜱传脑炎非风险区”的草场上，有一群全年在此放牧的山羊，该地区仅有零星的人类蜱传脑炎病例报告。在2008年10月至2009年12月期间，对这群山羊多次检测蜱传脑炎病毒（TBEV）特异性抗体和TBEV RNA。令人惊讶的是，在一只从未离开过该地区的山羊体内检测到了TBEV特异性抗体。为了将自然接触TBEV的结果与明确接触TBEV的结果进行比较，对两只山羊进行了实验性免疫。两只动物均产生了TBEV特异性抗体，但其中一只山羊产生抗体的时间延迟且抗体水平较低。此外，于2009年5月和6月从该草场采集了177只蜱，并通过实时RT-PCR进行检测。然而，未检测到TBEV RNA。结果表明，山羊可作为特定区域内TBEV的哨兵动物。为进一步验证这一观察结果，建议对大量动物进行进一步研究。