School of Pharmacy, Shanghai Jiao Tong University, 800th Dong-chuan Road, Shanghai 200240, China.
Int J Biol Macromol. 2011 Mar 1;48(2):375-80. doi: 10.1016/j.ijbiomac.2010.12.006. Epub 2010 Dec 13.
Novel water soluble phosphonium chitosan derivatives (WSPCSs) with two different degrees of substitution (3.6% and 4.2%) of quaternary phosphonium were synthesized in a homogeneous system at 25°C. The chemical structures of the WSPCS were characterized by (31)P NMR (nuclear magnetic resonance), (1)H NMR, FT-IR (Fourier-transformed infrared) spectroscopy and WAXD (wide-angle X-ray diffraction). Their solubility in water and several organic solvents and cytotoxicity to L929 cells were also evaluated. The degree of substitution (DS) of WSPCS was calculated by (1)H NMR. WAXD analysis showed that the derivatives had low crystallinity. The derivatives could be easily dissolved in water. MTT (methyl thiazolyl tetrazolium) assay indicated that they had low cytotoxicity to L929 cells.
新型水溶性磷鎓壳聚糖衍生物（WSPCSs）在 25°C 的均相体系中合成，取代度（DS）分别为 3.6%和 4.2%。（31）P NMR（核磁共振）、（1）H NMR、FT-IR（傅里叶变换红外）光谱和 WAXD（广角 X 射线衍射）对 WSPCS 的化学结构进行了表征。还评价了它们在水中和几种有机溶剂中的溶解度以及对 L929 细胞的细胞毒性。WSPCS 的取代度（DS）由（1）H NMR 计算。WAXD 分析表明，这些衍生物结晶度低。衍生物易溶于水。MTT（噻唑蓝）测定表明，它们对 L929 细胞的细胞毒性较低。
