School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2640-2645. doi: 10.1099/ijs.0.027987-0. Epub 2010 Dec 10.
A Gram-positive, aerobic bacterium, strain SA6(T), was isolated from marine sediment taken at a depth of 20 cm on the seashore of Lianyungang, Jiangsu Province, China. Strain SA6(T) contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids, and MK-10(H(2)) and MK-10(H(4)) as the predominant menaquinones. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Nocardiopsis. The almost-complete 16S rRNA gene sequence of strain SA6(T) was aligned with corresponding sequences of representatives of the genus Nocardiopsis and related taxa by using two tree-making algorithms. Strain SA6(T) formed a distinct phyletic line within the evolutionary radiation occupied by the genus Nocardiopsis and was related most closely to the type strain of Nocardiopsis lucentensis. Strain SA6(T) could be distinguished from its nearest phylogenetic relatives in the genus Nocardiopsis based on DNA-DNA relatedness data and a combination of phenotypic properties. Strain SA6(T) should therefore be assigned to the genus Nocardiopsis as a representative of a novel species, for which the name Nocardiopsis flavescens sp. nov. is proposed. The type strain is SA6(T) ( = CGMCC 4.5723(T) = JCM 17424(T)).
一株革兰氏阳性、需氧细菌，SA6(T) 株，从江苏省连云港市海滨 20 厘米深的海洋沉积物中分离得到。SA6(T) 株含有 meso-二氨基庚二酸，没有诊断糖，类型 PIII 磷脂，以及 MK-10(H(2)) 和 MK-10(H(4)) 作为主要的menaquinones。该菌表现出一系列与海洋放线菌属分类一致的化学和形态特性。SA6(T) 株的近完整 16S rRNA 基因序列与海洋放线菌属和相关分类群的代表菌株的相应序列进行比较，使用了两种树构建算法。SA6(T) 株在海洋放线菌属的进化辐射中形成了一个独特的系统发育谱系，与 Nocardiopsis lucentensis 模式株最为密切相关。根据 DNA-DNA 同源性数据和表型特性的综合分析，SA6(T) 株可与海洋放线菌属中最近的亲缘菌株区分开来。因此，SA6(T) 株应被归为海洋放线菌属的一个代表新种，命名为黄色放线菌（Nocardiopsis flavescens）sp. nov.。其模式株为 SA6(T) 株（=CGMCC 4.5723(T)=JCM 17424(T)）。
