Sabry Soraya A, Ghanem Nevine B, Abu-Ella Gehan A, Schumann Peter, Stackebrandt Erko, Kroppenstedt Reiner M
Alexandria University, Faculty of Science, Division: Microbiology, PO Box, Alexandria, Egypt.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):453-456. doi: 10.1099/ijs.0.02814-0.
An actinomycete, strain SNG49(T), was isolated from marine sediment of Abu Qir Bay, on the western seashore of Alexandria, Egypt. The bacterium was aerobic and Gram-positive. It produced beige to light-yellow aerial mycelium, brown substrate mycelium and straight to flexuous hyphae, but no specific spore chains. 16S rDNA sequence analysis and chemotaxonomic markers were consistent with classification of strain SNG49(T) in the genus Nocardiopsis, i.e. meso-diaminopimelic acid; no diagnostic sugars; phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol as polar lipids; menaquinones of the MK-10 series from MK-10(H(0)) to MK-10(H(8)); and iso/anteiso-branched and 10-methyl-branched fatty acids, the principal fatty acids being anteiso-17 : 0 and tuberculostearic acid. Nocardiopsis lucentensis and Nocardiopsis alba are the phylogenetic neighbours of strain SNG49(T), respectively showing 98.8 and 98.7 % 16S rRNA gene sequence similarity; however, moderate DNA-DNA reassociation values between these two species and strain SNG49(T) (44 and 60 %, respectively) showed that strain SNG49(T) could be clearly separated from them. These data, together with distinct physiological traits, led to the conclusion that this isolate represents a novel species within the genus Nocardiopsis, for which the name Nocardiopsis aegyptia is proposed. The type strain is SNG49(T) (=DSM 44442(T)=NRRL B-24244(T)).
从埃及亚历山大西部海岸阿布吉尔湾的海洋沉积物中分离出一株放线菌，菌株SNG49(T)。该菌为需氧型革兰氏阳性菌。它产生米色至浅黄色气生菌丝体、棕色基内菌丝体以及直的至弯曲的菌丝，但没有特定的孢子链。16S rDNA序列分析和化学分类标记与嗜诺卡氏菌属中菌株SNG49(T)的分类一致，即内消旋二氨基庚二酸；无诊断性糖；磷脂酰胆碱、磷脂酰甲基乙醇胺、磷脂酰肌醇、磷脂酰甘油和双磷脂酰甘油作为极性脂质；从MK-10(H(0))到MK-10(H(8))的MK-10系列甲基萘醌；以及异/anteiso-分支和10-甲基分支脂肪酸，主要脂肪酸为anteiso-17 : 0和结核硬脂酸。透明嗜诺卡氏菌和白色嗜诺卡氏菌分别是菌株SNG49(T)的系统发育近邻，16S rRNA基因序列相似性分别为98.8%和98.7%；然而，这两个物种与菌株SNG49(T)之间的中度DNA-DNA重结合值（分别为44%和60%）表明菌株SNG49(T)可与它们明显区分开。这些数据，连同独特的生理特性，得出结论：该分离株代表嗜诺卡氏菌属内的一个新物种，为此提出名为埃及嗜诺卡氏菌的名称。模式菌株为SNG49(T) (=DSM 44442(T)=NRRL B-24244(T))。
