跨越EcoRI底物位点的序列。

Garfin D E, Boyer H W, Goodman H M

Nucleic Acids Res. 1975 Oct;2(10):1851-65. doi: 10.1093/nar/2.10.1851.

Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.

通过分析各种DNA分子中酶切位点的核苷酸序列，对EcoRI限制性内切酶的底物识别进行了研究。5'-末端标记和同系色谱指纹图谱分析确定了一个跨越猴病毒40 DNA的EcoRI位点的17个碱基对的序列，以及一个与Col E1质粒DNA的EcoRI位点重叠的15个碱基对的序列。另外三种DNA也进行了类似的测试，不过在这些情况下没有确定延伸序列。结果表明，EcoRI位点是对称的双链，相当于-N-G-A-A-T-T-C-N-。