Rothel J S, Jones S L, Corner L A, Cox J C, Wood P R
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria.
Aust Vet J. 1990 Apr;67(4):134-7. doi: 10.1111/j.1751-0813.1990.tb07730.x.
An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma-interferon released in response to specific antigen in a whole blood culture system. The bio-assay previously described for the detection of bovine gamma-interferon (IFN-gamma) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN-gamma. The EIA detects less than 25pg/ml of recombinant bovine IFN-gamma and is specific for biologically active bovine IFN-gamma; and does not detect bovine alpha or beta interferon. IFN-gamma from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN-gamma EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.
最近开发了一种用于牛结核病的体外细胞检测方法。该检测方法可检测全血培养系统中因特定抗原而释放的γ-干扰素。先前描述的用于检测牛γ-干扰素（IFN-γ）的生物检测方法现已被一种夹心酶免疫测定法（EIA）所取代，该方法使用两种针对牛IFN-γ的单克隆抗体。该EIA可检测到低于25pg/ml的重组牛IFN-γ，且对具有生物活性的牛IFN-γ具有特异性；并且不检测牛α或β干扰素。绵羊、山羊和水牛的IFN-γ可被该EIA识别，但猪、鹿或人的IFN-γ则不能。牛IFN-γ EIA与全血培养系统结合使用时，已形成一种简单、快速且灵敏的体外检测方法，用于检测牛对牛分枝杆菌感染的特异性细胞介导免疫反应。
