Wood P R, Corner L A, Plackett P
Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Res Vet Sci. 1990 Jul;49(1):46-9.
Whereas in vitro assays of antibody responses to infectious agents are commonly used for routine diagnostic purposes, diagnostic tests for cellular responsiveness are confined to in vivo intradermal tests. This paper describes a simple and rapid in vitro cellular assay for bovine tuberculosis. The assay system is based on the detection of gamma interferon (gamma IFN), which is released in response to specific antigen. This assay can be carried out with whole blood samples thus avoiding the time-consuming task of isolating lymphocytes. A simple bioassay has been developed to quantitate the amount of gamma IFN produced, but this will eventually be replaced with an enzyme-linked immunoassay using monoclonal antibodies specific for bovine gamma IFN. The application of this system to bovine tuberculosis and other infectious diseases may provide a convenient in vitro cellular assay for routine diagnostic purposes.
虽然针对感染因子的抗体反应的体外检测通常用于常规诊断目的，但细胞反应性的诊断测试仅限于体内皮内试验。本文描述了一种用于牛结核病的简单快速的体外细胞检测方法。该检测系统基于γ干扰素（γIFN）的检测，γIFN是在对特定抗原作出反应时释放的。该检测可以用全血样本进行，从而避免了分离淋巴细胞这一耗时的任务。已经开发出一种简单的生物测定法来定量所产生的γIFN的量，但最终将被使用针对牛γIFN的单克隆抗体的酶联免疫测定法所取代。该系统在牛结核病和其他传染病中的应用可能为常规诊断目的提供一种方便的体外细胞检测方法。
