Washington University School of Medicine, St Louis, MO 63110, USA.
Am J Clin Nutr. 2011 Feb;93(2):402-12. doi: 10.3945/ajcn.110.005611. Epub 2010 Dec 15.
Loss of muscle mass with aging is a major public health concern. Omega-3 (n-3) fatty acids stimulate protein anabolism in animals and might therefore be useful for the treatment of sarcopenia. However, the effect of omega-3 fatty acids on human protein metabolism is unknown.
The objective of this study was to evaluate the effect of omega-3 fatty acid supplementation on the rate of muscle protein synthesis in older adults.
Sixteen healthy, older adults were randomly assigned to receive either omega-3 fatty acids or corn oil for 8 wk. The rate of muscle protein synthesis and the phosphorylation of key elements of the anabolic signaling pathway were evaluated before and after supplementation during basal, postabsorptive conditions and during a hyperaminoacidemic-hyperinsulinemic clamp.
Corn oil supplementation had no effect on the muscle protein synthesis rate and the extent of anabolic signaling element phosphorylation in muscle. Omega-3 fatty acid supplementation had no effect on the basal rate of muscle protein synthesis (mean ± SEM: 0.051 ± 0.005%/h compared with 0.053 ± 0.008%/h before and after supplementation, respectively; P = 0.80) but augmented the hyperaminoacidemia-hyperinsulinemia-induced increase in the rate of muscle protein synthesis (from 0.009 ± 0.005%/h above basal values to 0.031 ± 0.003%/h above basal values; P < 0.01), which was accompanied by greater increases in muscle mTOR(Ser2448) (P = 0.08) and p70s6k(Thr389) (P < 0.01) phosphorylation.
Omega-3 fatty acids stimulate muscle protein synthesis in older adults and may be useful for the prevention and treatment of sarcopenia. This trial was registered at clinical trials.gov as NCT00794079.
肌肉质量随年龄增长而减少是一个主要的公共健康问题。ω-3（n-3）脂肪酸可刺激动物的蛋白质合成代谢，因此可能对治疗肌肉减少症有用。然而，ω-3 脂肪酸对人类蛋白质代谢的影响尚不清楚。
本研究旨在评估 ω-3 脂肪酸补充对老年人肌肉蛋白质合成率的影响。
16 名健康的老年人被随机分配接受 ω-3 脂肪酸或玉米油补充 8 周。在补充前后的基础、吸收后状态下以及在高氨基酸血症-高胰岛素血症钳夹期间，评估肌肉蛋白质合成率和合成代谢信号通路关键元素的磷酸化。
玉米油补充对肌肉蛋白质合成率和肌肉中合成代谢信号元件磷酸化程度均无影响。ω-3 脂肪酸补充对基础肌肉蛋白质合成率无影响（平均值±SEM：分别为 0.051±0.005%/h 和 0.053±0.008%/h；P=0.80），但增加了高氨基酸血症-高胰岛素血症引起的肌肉蛋白质合成率的增加（从基础值以上的 0.009±0.005%/h 增加到基础值以上的 0.031±0.003%/h；P<0.01），同时肌肉 mTOR（Ser2448）（P=0.08）和 p70s6k（Thr389）（P<0.01）磷酸化也增加。
ω-3 脂肪酸可刺激老年人的肌肉蛋白质合成，可能对预防和治疗肌肉减少症有用。该试验在 clinicaltrials.gov 上注册为 NCT00794079。
