由于缺乏 mecA 基因的葡萄球菌盒式染色体元件的存在，使用快速商业 PCR 检测法会导致耐甲氧西林金黄色葡萄球菌携带者的错误鉴定比例很高。
High proportion of wrongly identified methicillin-resistant Staphylococcus aureus carriers by use of a rapid commercial PCR assay due to presence of staphylococcal cassette chromosome element lacking the mecA gene.
机构信息
Hospital Preventive Medicine Service, Institute of Microbiology, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland.
出版信息
J Clin Microbiol. 2011 Feb;49(2):722-4. doi: 10.1128/JCM.01988-10. Epub 2010 Dec 15.
Abstract
During a 9-month period, 217 patients were newly diagnosed as methicillin-resistant Staphylococcus aureus (MRSA) carriers by using a commercial rapid PCR-based test (GeneXpert). However, no MRSA was recovered by culturing the second swab in 61 of these patients. Further analyses showed that 28 (12.9%) of the patients harbored S. aureus isolates with a staphylococcal cassette chromosome element lacking the mecA gene and were thus incorrectly determined to be MRSA carriers.
摘要
在 9 个月期间，使用一种商业性的快速基于 PCR 的检测（GeneXpert），217 位患者被新诊断为耐甲氧西林金黄色葡萄球菌（MRSA）携带者。然而，在这 61 位患者中，通过培养第二份拭子，没有回收 MRSA。进一步分析表明，28 位（12.9%）患者携带缺乏 mecA 基因的葡萄球菌盒染色体元件的金黄色葡萄球菌分离株，因此被错误地确定为 MRSA 携带者。
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