Anbazhagan Ganesh Kumar, Krishnamoorthy Sridharan, Thiyagarajan Thirunalasundari
Ganesh Kumar Anbazhagan, Sridharan Krishnamoorthy, Thirunalasundari Thiyagarajan 620024, Tamil Nadu, India.
World J Hepatol. 2010 Jan 27;2(1):42-8. doi: 10.4254/wjh.v2.i1.42.
To determine the seroprevalence of hepatitis C virus (HCV) and its co-infection with hepatitis B virus (HBV), hepatitis delta agent (HDV) and human immunodeficiency virus (HIV) among liver disease patients of south Tamil Nadu.
A total of 1012 samples comprising 512 clinically diagnosed cases of liver disease patients and 500 apparently healthy age and sex matched individuals were screened for Hepatitis C virus (anti HCV and HCV RNA), Hepatitis B virus (HBsAg), Hepatitis delta agent (anti HDV) and Human immuno virus (antibodies to HIV-1 and HIV-2) using commercially available enzyme linked immunosorbent assay kits. HCV RNA was detected by RT-PCR. Liver function tests like ALT, AST, GGT, ALP, bilirubin and albumin were also studied.
The seroprevalence of HCV was found to be 5.6% among liver disease patients by ELISA. 27/512, 49/512 and 12/512 patients were positive for HIV, HBV & HDV respectively. Co-infection of HCV & HBV was found in 8 patients, with 6 for HCV & HIV and 4 for HCV, HBV & HIV co-infections. Sex-wise analysis showed that HIV, HCV & HBV and HCV & HIV co-infection was high among females whereas for HBV it was high in males. The mean ALT and AST in HCV positive cases were 42.1 ± 8.3 and 49 ± 10.1. In people co-infected with HCV & HBV or HCV & HIV or HCV, HBV & HIV the mean ALT of 58.0 ± 03.16, 56.78 ± 4.401 and 64.37 ± 4.01 respectively.
We strongly recommend routine test of the blood for HCV in addition to HBV and HIV. We also recommend individualized counseling to identify those at risk and testing for those who want it. Improved surveillance and periodic epidemiological studies will have to be undertaken to monitor and prevent these blood-borne viruses.
确定印度泰米尔纳德邦南部肝病患者中丙型肝炎病毒（HCV）的血清流行率及其与乙型肝炎病毒（HBV）、丁型肝炎病毒（HDV）和人类免疫缺陷病毒（HIV）的合并感染情况。
使用市售酶联免疫吸附测定试剂盒，对总共1012份样本进行筛查，其中包括512例临床诊断的肝病患者样本以及500例年龄和性别匹配的明显健康个体样本，检测项目包括丙型肝炎病毒（抗HCV和HCV RNA）、乙型肝炎病毒（HBsAg）、丁型肝炎病毒（抗HDV）和人类免疫病毒（抗HIV - 1和抗HIV - 2抗体）。通过逆转录聚合酶链反应（RT - PCR）检测HCV RNA。还研究了肝功能指标如谷丙转氨酶（ALT）、谷草转氨酶（AST）、γ-谷氨酰转肽酶（GGT）、碱性磷酸酶（ALP）、胆红素和白蛋白。
通过酶联免疫吸附测定法发现，肝病患者中HCV的血清流行率为5.6%。分别有27/512、49/512和12/512例患者HIV、HBV和HDV检测呈阳性。发现8例患者HCV与HBV合并感染，6例患者HCV与HIV合并感染，4例患者HCV、HBV和HIV合并感染。按性别分析显示，女性中HIV、HCV与HBV以及HCV与HIV合并感染率较高，而男性中HBV感染率较高。HCV阳性病例的平均ALT和AST分别为42.1±8.3和49±10.1。在HCV与HBV或HCV与HIV或HCV、HBV与HIV合并感染的人群中，平均ALT分别为58.0±3.16、56.78±4.401和64.37±4.01。
我们强烈建议除了检测HBV和HIV外，对血液进行HCV常规检测。我们还建议进行个性化咨询，以识别高危人群并为有需求者进行检测。必须加强监测和定期开展流行病学研究，以监测和预防这些血源性病毒。
