Department of Biotechnology, Integral University, Lucknow 226026, Uttar Pradesh, India.
Mol Biol Rep. 2011 Nov;38(8):5037-53. doi: 10.1007/s11033-010-0650-9. Epub 2010 Dec 16.
The Dof (DNA binding with One Finger) family represents a classic zinc-finger transcription factors involved with multifarious roles exclusively in plants. There exists great diversity in terms of number of Dof genes observed in different crops. In current study, a total of 28 putative Dof genes have been predicted in silico from the recently available whole genome shotgun sequence of Sorghum bicolor (L.) Moench (with assigned accession numbers TPA:BK006983-BK007006 and TPA:BK007079-BK007082). The predicted SbDof genes are distributed on nine out of ten chromosomes of sorghum and most of these genes lack introns based on canonical intron/exon structure. Phylogenetic analysis of 28 SbDof proteins resulted in four subgroups constituting six clusters. The comparative phylogenetic analysis of these Dof proteins along with 30 rice and 36 Arabidopsis Dof proteins revealed six major groups similar to what has been observed earlier for rice and Arabidopsis. Motif analysis revealed the presence of conserved 50-52 amino acids Dof domain uniformly distributed across all the 28 Dof proteins of sorghum. The in silico cis-regulatory elements analysis of these SbDof genes suggested its diverse functions associated with light responsiveness, endosperm specific gene expression, hormone responsiveness, meristem specific expression and stress responsiveness.
Dof（与一个手指结合的 DNA 结合）家族代表了一类经典的锌指转录因子，专门在植物中发挥多种作用。在不同作物中观察到的 Dof 基因数量存在很大的多样性。在当前的研究中，从最近获得的 Sorghum bicolor（L.）Moench 的全基因组鸟枪法序列中（分配的登录号为 TPA:BK006983-BK007006 和 TPA:BK007079-BK007082），通过计算机预测了总共 28 个假定的 Dof 基因。预测的 SbDof 基因分布在高粱的十条染色体中的九条上，并且大多数这些基因缺乏基于规范的内含子/外显子结构的内含子。28 个 SbDof 蛋白的系统发育分析导致了四个亚组，构成了六个簇。对这些 Dof 蛋白与 30 个水稻和 36 个拟南芥 Dof 蛋白的比较系统发育分析表明，存在六个主要的组，与之前对水稻和拟南芥的观察结果相似。基序分析表明，存在保守的 50-52 个氨基酸 Dof 结构域，均匀分布在高粱的所有 28 个 Dof 蛋白中。这些 SbDof 基因的计算机顺式调控元件分析表明，它们具有与光反应、胚乳特异性基因表达、激素反应、分生组织特异性表达和应激反应相关的多种功能。
