Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, 191 Auditorium Road, Storrs, CT 06269-3222, USA.
Anal Bioanal Chem. 2011 Feb;399(4):1591-9. doi: 10.1007/s00216-010-4559-x. Epub 2010 Dec 15.
Nucleic acid aptamers have recently attracted significant attention in the field of clinical diagnosis because they have numerous merits, such as high affinity, high specificity, small size, little immunogenicity, stable structures, and ease of synthesis. This review focuses on discussing the potential applications of aptamers in cell detection and molecular imaging. For the ex vivo cell detection, this review discusses the status of five strategies: endogenous nucleic acid analysis, flow cytometry analysis, nanoparticle-based cell sensing, microfluidic cell separation, and histological examination. This review also discusses in vivo molecular and cell imaging by introducing aptamer-based molecular imaging, cell imaging, and integrated imaging and therapy. On the basis of the status of these promising studies, this review summarizes several challenging issues and unmet needs that may require more effort or attention in the future.
核酸适体最近在临床诊断领域引起了广泛关注，因为它们具有许多优点，如高亲和力、高特异性、体积小、免疫原性低、结构稳定、易于合成。本综述重点讨论了适体在细胞检测和分子成像中的潜在应用。对于细胞的体外检测，本综述讨论了五种策略的现状：内源性核酸分析、流式细胞术分析、基于纳米颗粒的细胞传感、微流控细胞分离和组织学检查。本综述还通过介绍基于适体的分子成像、细胞成像和集成成像与治疗，讨论了体内分子和细胞成像。在这些有前途的研究现状的基础上，本综述总结了一些具有挑战性的问题和未满足的需求，这些问题可能需要在未来投入更多的努力或关注。
