School of Chemistry, Monash University, Clayton, VIC 3800, Australia.
Eur Biophys J. 2011 Apr;40(4):437-46. doi: 10.1007/s00249-010-0652-5. Epub 2010 Dec 16.
The increasing prevalence of antibiotic-resistant bacteria is becoming a public health crisis. Antimicrobial peptides (AMPs) are a promising solution, because bacterial resistance is less likely. Quartz crystal microbalance with dissipation monitoring (QCM-D) is a versatile and valuable technique for investigation of these peptides. This article looks at the different approaches to the interpretation of QCM-D data, showing how to extract the maximum information from the data. Five AMPs of diverse charge, length and activity are used as case studies: caerin 1.1 wild-type, two caerin 1.1 mutants (Gly15Gly19-caerin 1.1 and Ala15Ala19-caerin 1.1), aurein 1.2 and oncocin. The interaction between the AMP and a 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membrane is analysed inter alia using frequency-dissipation plots (∆f-∆D plots) to ascertain the mechanism of action of the AMP. The ∆f-∆D plot can then be used to provide a fingerprint for the AMP-membrane interaction. Building up a database of these fingerprints for all known AMPs will enable the relationship between AMP structure and membrane activity to be better understood, hopefully leading to the future development of antibiotics without bacterial resistance.
抗生素耐药菌的日益流行正在成为公共健康危机。抗菌肽 (AMPs) 是一种很有前途的解决方案，因为细菌耐药性的可能性较小。石英晶体微天平耗散监测 (QCM-D) 是一种用于研究这些肽的多功能且有价值的技术。本文探讨了解释 QCM-D 数据的不同方法，展示了如何从数据中提取最大信息。使用了 charge、length 和 activity 不同的五种 AMP 作为案例研究：野生型 caerin 1.1、两种 caerin 1.1 突变体 (Gly15Gly19-caerin 1.1 和 Ala15Ala19-caerin 1.1)、aurein 1.2 和 oncocin。使用频率耗散图 (∆f-∆D 图) 等方法分析 AMP 与 1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱 (DMPC) 膜之间的相互作用，以确定 AMP 的作用机制。然后可以使用 ∆f-∆D 图为 AMP-膜相互作用提供指纹。为所有已知的 AMP 建立这些指纹的数据库将使我们能够更好地理解 AMP 结构与膜活性之间的关系，希望能为未来开发无细菌耐药性的抗生素提供帮助。
