DI Biao, Bai Zhi-jun, Wang Yu-lin, Luo Lei, Chen Yu, Jiang Li-yun, Yang Zhi-cong, Wang Ming
Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jul;31(7):804-7.
To analyze and trace the infection source the envelope (E) gene of the new emerged type 3 dengue virus in Guangzhou in 2009.
Sera were collected from patients infected with local dengue fever. Dengue virus was cultured and isolated by C6/36 cells. The whole length E gene was amplified from the positive specimen by RT-PCR, thereby sequenced and phylogenetic tree drawn by neighbor-joining method. Both data on epidemiologic and molecular studies were processed and analysed.
7 strains of type 3 dengue virus were isolated from samples of the 19 patients. E gene of these strains was amplified. The complete E genes of 7 strains belonged to 1479 nucleotides in length, encoding a polyprotein of 493 amino acids. Data from the phylogenetic analysis showed that 09/GZ/1081, 09/GZ/1483 and 09/GZ/10806 strains fell within the Southeast Asia/South Pacific group. 09/GZ/10616, 09/GZ/11144, 09/GZ/11194 while 09/GZ/13105 strains fell within the India group.
The type 3 dengue virus identified in Guangzhou area in 2009 was imported and could be decided into two genotypes.
分析并追踪2009年广州新出现的3型登革病毒包膜（E）基因的感染源。
采集本地登革热感染患者的血清。用C6/36细胞培养并分离登革病毒。通过RT-PCR从阳性标本中扩增全长E基因，进而进行测序并采用邻接法绘制系统发育树。对流行病学和分子研究数据进行处理与分析。
从19例患者的样本中分离出7株3型登革病毒。扩增了这些毒株的E基因。7株毒株的完整E基因长度均为1479个核苷酸，编码一个由493个氨基酸组成的多聚蛋白。系统发育分析数据显示，09/GZ/1081、09/GZ/1483和09/GZ/10806毒株属于东南亚/南太平洋组。09/GZ/10616、09/GZ/11144、09/GZ/11194以及09/GZ/13105毒株属于印度组。
2009年在广州地区鉴定出的3型登革病毒为输入性，可分为两个基因型。
