Yousefizadeh Shahnaz, Farkhondeh Tahereh, Samarghandian Saeed
Department of Laboratory and Clinical Sciences, Faculty of Para-Veterinary, Ilam University, Ilam, Iran.
Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Curr Aging Sci. 2019;12(1):49-54. doi: 10.2174/1874609812666190416160918.
Diabetes and its complications are age-related diseases. Low-grade inflammation plays the main role in the aging processes. Diazinon (DZN), an organophosphate pesticide, has been found to induce metabolic disturbances.
The present study was designed to investigate the impact of DZN on age-related changes on inflammatory cells, blood glucose concentration, lipid profile, and liver and kidney function indices in adult and aged rats.
Male rats (2 and 16 month old) were orally administrated with DZN (15 mg/kg) for 4 weeks. Then the blood was obtained for measuring inflammatory cells, lipid profile, glucose and serum biochemical indices such as liver enzymes, albumin, total protein, creatinine (Cr), urea, and uric acid in the serum of adult and aged male rats.
DZN increased the blood levels of glucose and the percentage of lymphocytes and also serum levels of TChol, TG, LDL-c, AST, ALT, ALP, LDH, Cr, urea, and uric acid in the adult and aged rats versus the aged matched control rats (p< 0.001). A marked reduction in HDL-c levels, total protein, albumin, and in the percentage of neutrophils were seen in the adult and aged animals exposed to DZN versus the aged matched control rats. DZN also increased the levels of LDL-c and ALT in the aged rats versus adult animals.
The present study indicated that DZN can cause metabolic disturbance. However, the age-dependent effects of DZN on metabolic indices were not be confirmed by the present data.
糖尿病及其并发症是与年龄相关的疾病。低度炎症在衰老过程中起主要作用。二嗪农（DZN），一种有机磷农药，已被发现可诱发代谢紊乱。
本研究旨在调查DZN对成年和老年大鼠炎症细胞、血糖浓度、血脂谱以及肝肾功能指标的年龄相关变化的影响。
对雄性大鼠（2月龄和16月龄）口服给予DZN（15毫克/千克），持续4周。然后采集血液，测量成年和老年雄性大鼠血清中的炎症细胞、血脂谱、葡萄糖以及血清生化指标，如肝酶、白蛋白、总蛋白、肌酐（Cr）、尿素和尿酸。
与年龄匹配的对照大鼠相比，DZN使成年和老年大鼠的血糖水平、淋巴细胞百分比以及血清总胆固醇（TChol）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、肌酐（Cr）、尿素和尿酸水平升高（p<0.001）。与年龄匹配的对照大鼠相比，暴露于DZN的成年和老年动物的高密度脂蛋白胆固醇（HDL-c）水平、总蛋白、白蛋白以及中性粒细胞百分比显著降低。与成年动物相比，DZN还使老年大鼠的LDL-c和ALT水平升高。
本研究表明DZN可导致代谢紊乱。然而，本数据未证实DZN对代谢指标的年龄依赖性影响。
