Wang Youmin, Bish Sheldon, Tunnell James W, Zhang Xiaojing
Microelectronics Research Center, Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, 78758 USA.
Opt Express. 2010 Nov 8;18(23):24101-8. doi: 10.1364/OE.18.024101.
We demonstrate a hyperspectral and depth sensitive diffuse optical imaging microsystem, where fast scanning is provided by a CMOS compatible 2-axis MEMS mirror. By using lissajous scanning patterns, large field-of-view (FOV) of 1.2 cmx1.2 cm images with lateral resolution of 100 µm can be taken at 1.3 frames-per-second (fps). Hyperspectral and depth-sensitive images were acquired on tissue simulating phantom samples containing quantum dots (QDs) patterned at various depths in Polydimethylsiloxane (PDMS). Device performance delivers 6 nm spectral resolution and 0.43 wavelengths per second acquisition speed. A sample of porcine epithelium with subcutaneously placed QDs was also imaged. Images of the biological sample were processed by spectral unmixing in order to qualitatively separate chromophores in the final images and demonstrate spectral performance of the imaging system.
我们展示了一种高光谱和深度敏感的漫射光学成像微系统，其中快速扫描由CMOS兼容的双轴MEMS镜提供。通过使用李萨如图形扫描模式，每秒1.3帧（fps）的速度下可拍摄1.2 cmx1.2 cm、横向分辨率为100 µm的大视野（FOV）图像。在含有在聚二甲基硅氧烷（PDMS）中不同深度图案化的量子点（QD）的组织模拟体模样品上采集了高光谱和深度敏感图像。该设备性能提供6 nm的光谱分辨率和每秒0.43个波长的采集速度。还对皮下放置有量子点的猪上皮样本进行了成像。通过光谱解混处理生物样本的图像，以便在最终图像中定性分离发色团，并展示成像系统的光谱性能。
