Department of Endodontics, Faculty of Odontology, Malmö University, Malmö, Sweden.
Int Endod J. 2011 Mar;44(3):259-67. doi: 10.1111/j.1365-2591.2010.01824.x. Epub 2010 Dec 17.
To characterize the hard tissue formed in human teeth experimentally pulp capped either with calcium hydroxide or with Emdogain Gel (Biora AB, Malmö, Sweden) - a derivative of enamel matrix (EMD), using two markers for dentine; dentine sialoprotein (DSP) and type 1 collagen (Col I).
Affinity-purified rabbit anti-Col I and anti-DSP polyclonal antibodies were used to stain histological sections from nine pairs of contra-lateral premolars that had been experimentally pulp amputated and randomly capped with EMDgel or calcium hydroxide. Twelve weeks after the teeth had been pulp capped, they were extracted, fixed, demineralized and serially sectioned prior to immunohistochemical staining.
In the calcium hydroxide treated teeth DSP was seen in the new hard tissue which formed a bridge. DSP was also seen in the newly formed hard tissue in the EMDgel-treated teeth. Proliferated pulp tissue partly filled the space initially occupied by EMDgel and DSP-stained hard tissue was observed alongside exposed dentine surfaces as well as in isolated masses within the proliferated pulp tissue, although the new hard tissue did not cover the pulp exposure. DSP staining was also seen in the cells lining the hard tissue in both groups. Col I staining was seen in the newly formed hard tissue in both groups.
The new hard tissue formed after pulp capping with EMDgel or calcium hydroxide contained DSP and Col I, considered to be markers for dentine. Thus, the newly formed hard tissue can be characterized as dentine rather than unspecific hard tissue.
使用两种牙本质标志物——牙本质涎磷蛋白（DSP）和 I 型胶原（Col I），对用氢氧化钙或 Emdogain Gel（Biora AB，马尔默，瑞典）——一种釉基质衍生物质（EMD）盖髓的人牙齿中的硬组织进行特征描述。
用亲和纯化的兔抗 Col I 和抗 DSP 多克隆抗体对 9 对对侧前磨牙的组织学切片进行染色，这些牙齿已经被实验性牙髓切断并随机用 EMDgel 或氢氧化钙盖髓。在盖髓后 12 周，将牙齿拔出、固定、脱矿并连续切片，然后进行免疫组织化学染色。
在氢氧化钙处理的牙齿中，新形成的桥状硬组织中可见 DSP。在 EMDgel 处理的牙齿中，新形成的硬组织中也可见 DSP。增生的牙髓组织部分填充了最初被 EMDgel 占据的空间，在暴露的牙本质表面以及增生的牙髓组织内的孤立团块中观察到 DSP 染色的硬组织，尽管新的硬组织没有覆盖牙髓暴露。在两组中，DSP 染色也见于硬组织衬里细胞。两组中均可见 Col I 染色在新形成的硬组织中。
用 EMDgel 或氢氧化钙盖髓后形成的新硬组织含有 DSP 和 Col I，被认为是牙本质的标志物。因此，新形成的硬组织可以被描述为牙本质，而不是非特异性硬组织。
