基于超灵敏纳米金探针的免疫层析法用于同时检测花生中的总黄曲霉毒素。
Ultrasensitive nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins in peanuts.
机构信息
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
出版信息
Biosens Bioelectron. 2011 Feb 15;26(6):2877-82. doi: 10.1016/j.bios.2010.11.031. Epub 2010 Dec 1.
An ultrasensitive immunochromatographic (IC) assay for simultaneous detection of total aflatoxins (AFB1, AFB2, AFG1, and AFG2) was developed to meet the requirement for rapidly monitoring aflatoxins in agro-products. The assay was based on a competitive format and its sensitivity was improved by using a novel criterion to screen the optimal amount of monoclonal antibody (MAb) labeled to nanogold particles. The visual detection limits (VDLs) for aflatoxins B1, B2, G1, and G2 in peanut matrix were 0.03, 0.06, 0.12, and 0.25 ng mL(-1), respectively, which were lower than those of published literatures. The results of IC assay were in good agreement with those of high performance liquid chromatography (HPLC) in the analysis of aflatoxins in peanuts, demonstrating the practical applicability of the developed assay in real samples. This qualitative test based on the visual evaluation of results did not require any equipment. Overall, to our knowledge, this is the first report of qualitative detection for total aflatoxins by immunochromatographic assay.
一种超灵敏的免疫层析（IC）分析方法被开发出来，用于同时检测总黄曲霉毒素（AFB1、AFB2、AFG1 和 AFG2），以满足快速监测农产品中黄曲霉毒素的要求。该分析方法基于竞争模式，并通过使用一种新的标准来筛选最佳量的标记纳米金颗粒的单克隆抗体（MAb），从而提高了其灵敏度。在花生基质中，黄曲霉毒素 B1、B2、G1 和 G2 的视觉检测限（VDL）分别为 0.03、0.06、0.12 和 0.25 ng mL(-1)，均低于已发表文献中的值。IC 分析的结果与高效液相色谱（HPLC）在花生中分析黄曲霉毒素的结果非常吻合，证明了该方法在实际样品中的实际适用性。这种基于结果的视觉评估的定性测试不需要任何设备。总的来说，据我们所知，这是首次报道用免疫层析法进行总黄曲霉毒素的定性检测。
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