University of Regensburg, Institute for Microbiology and Archaea Centre, Universitätsstrasse 31, 93053 Regensburg, Germany.
Appl Environ Microbiol. 2011 Mar;77(5):1556-62. doi: 10.1128/AEM.02423-10. Epub 2010 Dec 17.
The surfaces of 8 bacterial and 23 archaeal species, including many hyperthermophilic Archaea, could be stained using succinimidyl esters of fluorescent dyes. This allowed us for the first time to analyze the mode of cell wall growth in Archaea by subculturing stained cells. The data obtained show that incorporation of new cell wall material in Archaea follows the pattern observed for Bacteria: in the coccoid species Pyrococcus furiosus incorporation was in the region of septum formation while for the rod-shaped species Methanopyrus kandleri and Methanothermus sociabilis, a diffuse incorporation of cell wall material over the cell length was observed. Cell surface appendages like fimbriae/pili, fibers, or flagella were detectable by fluorescence staining only in a very few cases although their presence was proven by electron microscopy. Our data in addition prove that Alexa Fluor dyes can be used for in situ analyses at temperatures up to 100°C.
8 种细菌和 23 种古菌的表面，包括许多高温古菌，可以用荧光染料的琥珀酰亚胺酯进行染色。这使我们第一次能够通过培养染色细胞来分析古菌细胞壁生长的模式。获得的数据表明，古菌中新细胞壁物质的掺入遵循在细菌中观察到的模式：在球形种 Pyrococcus furiosus 中，掺入发生在隔膜形成区域，而对于杆状种 Methanopyrus kandleri 和 Methanothermus sociabilis，则观察到细胞壁物质在整个细胞长度上的弥散掺入。尽管电子显微镜证明了它们的存在，但只有在极少数情况下，通过荧光染色才能检测到菌毛/纤毛、纤维或鞭毛等细胞表面附属物。此外，我们的数据还证明，Alexa Fluor 染料可用于高达 100°C 的原位分析。
