Murphy Gavin E, Jensen Grant J
California Institute of Technology, Pasadena, CA, USA.
Biotechniques. 2007 Oct;43(4):413, 415, 417 passim. doi: 10.2144/000112568.
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
电子冷冻断层扫描是一种新兴技术，它能够对单个大分子、病毒乃至小型完整细胞等独特生物对象的结构进行三维（3-D）重建，使其接近天然状态，分辨率约为5纳米。本文简要总结了所需的仪器设备、样品制备及其局限性、数据收集、典型结果以及未来前景。
