Sreejai R, Jaya D S
Department of Environmental Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram - 695 581, Kerala, India.
Toxicol Int. 2010 Jul;17(2):71-7. doi: 10.4103/0971-6580.72674.
In the present aquarium study, Oreochromis mossambicus Peters were exposed to two different concentrations of hydrogen sulfide (H(2)S) (4.9 and 6.6 mg/l), and the changes in lipid peroxidation (LP) products and antioxidants in test fishes were determined in time intervals of 12, 24, 48, 72, and 96 hours. The results showed that with respect to the H(2)S concentration and duration of exposure, alterations were observed in the concentration of LP products and antioxidants in the various organs of the test fishes. Malondialdehyde (MDA) content increased in the liver, gill, kidney, and brain on exposure to H(2)S up to 48 hours, and then the MDA content showed steady value up to 98 hours experimental period. Brain and kidney of fishes showed the maximum increase in concentration of reduced glutathione (GSH) on H(2)S treatment. The gradual decrease in concentration of GSH in the tissues of H(2) S-exposed fishes after 48 to 96 hours compared with the control shows the loss of adaptive mechanisms and the oxidation of GSH to glutathione disulphide (GSSG). Slight increase in the activity of GSH-S-transferase and decrease in activity of GSH peroxidase demonstrated the incapability of the vital organs in neutralizing the peroxides generated in the oxidative stress condition.
在当前的水族箱研究中，将莫桑比克罗非鱼暴露于两种不同浓度的硫化氢（H₂S）（4.9毫克/升和6.6毫克/升）中，并在12、24、48、72和96小时的时间间隔内测定受试鱼体内脂质过氧化（LP）产物和抗氧化剂的变化。结果表明，随着H₂S浓度和暴露时间的变化，受试鱼各器官中LP产物和抗氧化剂的浓度出现了改变。暴露于H₂S长达48小时时，肝脏、鳃、肾脏和大脑中的丙二醛（MDA）含量增加，然后在长达98小时的实验期内MDA含量呈现稳定值。经H₂S处理后，鱼的大脑和肾脏中还原型谷胱甘肽（GSH）浓度增加最多。与对照组相比，暴露于H₂S的鱼的组织中GSH浓度在48至96小时后逐渐降低，这表明其适应性机制丧失，且GSH被氧化为谷胱甘肽二硫化物（GSSG）。谷胱甘肽S-转移酶活性略有增加，而谷胱甘肽过氧化物酶活性降低，这表明重要器官无法中和氧化应激条件下产生的过氧化物。
