Yubero-Serrano Elena M, Delgado-Casado Nieves, Delgado-Lista Javier, Perez-Martinez Pablo, Tasset-Cuevas Inmaculada, Santos-Gonzalez Monica, Caballero Javier, Garcia-Rios Antonio, Marin Carmen, Gutierrez-Mariscal Francisco M, Fuentes Francisco, Villalba Jose M, Tunez Isaac, Perez-Jimenez Francisco, Lopez-Miranda Jose
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba and CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Spain.
Age (Dordr). 2011 Dec;33(4):579-90. doi: 10.1007/s11357-010-9199-8. Epub 2010 Dec 18.
Postprandial oxidative stress is characterized by an increased susceptibility of the organism towards oxidative damage after consumption of a meal rich in lipids and/or carbohydrates. We have investigated whether the quality of dietary fat alters postprandial cellular oxidative stress and whether the supplementation with coenzyme Q(10) (CoQ) lowers postprandial oxidative stress in an elderly population. In this randomized crossover study, 20 participants were assigned to receive three isocaloric diets for periods of 4 week each: (1) Mediterranean diet supplemented with CoQ (Med+CoQ diet), (2) Mediterranean diet (Med diet), and (3) saturated fatty acid-rich diet (SFA diet). After a 12-h fast, the volunteers consumed a breakfast with a fat composition similar to that consumed in each of the diets. CoQ, lipid peroxides (LPO), oxidized low-density lipoprotein (oxLDL), protein carbonyl (PC), total nitrite, nitrotyrosine plasma levels, catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities and ischemic reactive hyperaemia (IRH) were determined. Med diet produced a lower postprandial GPx activity and a lower decrease in total nitrite level compared to the SFA diet. Med and Med+CoQ diets induced a higher postprandial increase in IRH and a lower postprandial LPO, oxLDL, and nitrotyrosine plasma levels than the SFA diet. Moreover, the Med+CoQ diet produced a lower postprandial decrease in total nitrite and a greater decrease in PC levels compared to the other two diets and lower SOD, CAT, and GPx activities than the SFA diet.In conclusion, Med diet reduces postprandial oxidative stress by reducing processes of cellular oxidation and increases the action of the antioxidant system in elderly persons and the administration of CoQ further improves this redox balance.
餐后氧化应激的特征是，在摄入富含脂质和/或碳水化合物的膳食后，机体对氧化损伤的易感性增加。我们研究了膳食脂肪的质量是否会改变餐后细胞氧化应激，以及补充辅酶Q(10)(CoQ)是否会降低老年人群的餐后氧化应激。在这项随机交叉研究中，20名参与者被分配接受三种等热量饮食，每种饮食为期4周：(1)补充CoQ的地中海饮食(Med+CoQ饮食)，(2)地中海饮食(Med饮食)，以及(3)富含饱和脂肪酸的饮食(SFA饮食)。在禁食12小时后，志愿者食用了一份脂肪成分与每种饮食中所摄入脂肪成分相似的早餐。测定了CoQ、脂质过氧化物(LPO)、氧化型低密度脂蛋白(oxLDL)、蛋白质羰基(PC)、总亚硝酸盐含量、硝基酪氨酸血浆水平、过氧化氢酶、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)活性以及缺血性反应性充血(IRH)。与SFA饮食相比，Med饮食产生的餐后GPx活性较低，总亚硝酸盐水平下降幅度较小。与SFA饮食相比，Med饮食和Med+CoQ饮食诱导的餐后IRH升高幅度更大，餐后LPO、oxLDL和硝基酪氨酸血浆水平更低。此外，与其他两种饮食相比，Med+CoQ饮食导致餐后总亚硝酸盐的下降幅度更小，PC水平的下降幅度更大，并且与SFA饮食相比，其SOD、CAT和GPx活性更低。总之，Med饮食通过减少细胞氧化过程来降低餐后氧化应激，并增强老年人抗氧化系统的作用，而补充CoQ进一步改善了这种氧化还原平衡。
