Salzberg Steven L
Center for Bioinformatics and Computational Biology and Department of Computer Science, University of Maryland College Park, MD 20742 USA.
F1000 Biol Rep. 2010 Aug 19;2:64. doi: 10.3410/B2-64.
The latest high-throughput DNA sequencing technology can now be applied on a large scale to capture the complete set of mRNA transcripts in a cell, using a technique called RNA-seq. Although RNA-seq is only 2 years old, it has rapidly swept through the field of genomics, and it is now being used to analyze the transcriptomes of organisms ranging from bacteria to primates. The depth of sequencing allows researchers to quantify the level of expression of genes, to discover alternative isoforms in eukaryotic species, and even to characterize the operon structure of bacterial genomes.
最新的高通量DNA测序技术现在可以大规模应用，通过一种叫做RNA测序（RNA-seq）的技术来捕获细胞中的全套mRNA转录本。尽管RNA测序技术问世才两年，但它已迅速席卷基因组学领域，目前正被用于分析从细菌到灵长类动物等各种生物体的转录组。测序深度使研究人员能够量化基因的表达水平，发现真核生物中的可变异构体，甚至能够描绘细菌基因组的操纵子结构。
