Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 100875 Beijing, China.
J Environ Sci (China). 2010;22(9):1435-40. doi: 10.1016/s1001-0742(09)60272-2.
Nonylphenol (NP), a xenoestrogen ubiquitously found in aquatic ecosystems, is of high environmental concern. The present work assessed the effects of exposure to NP on locomotor activity and social behavior in male and female zebrafish (Danio rerio), to evaluate the possible hazard of NP to fish behavior and to pursue a potential biomarker of NP contamination. Fish were randomly divided into six groups. Five groups were receiving 0.1, 1, 10, 50 and 100 microg/L nominal concentrations of NP for 60 days, respectively. A sixth control group was given the same treatment as the other five groups, but no NP. Locomotor activity, aggressive behavior, group preference and leaving shoal were examined. NP exposure showed marked influence on locomotor activity of the male zebrafish, whereas that of the female was not significantly affected by NP. Aggressive behavior and group preference were significantly affected by NP exposure in both male and female groups. Locomotor activity and aggressive behavior of the male and group preference of both male and female zebrafish were clearly inhibited at 100 microg/L NP. No significant alteration in leaving shoal was observed under NP exposure. The results suggested that changes in locomotor activity and aggressive behavior of the male and group preference of both male and female may be used as an ecologically relevant integrative biomarker of NP contamination.
壬基酚（NP）是一种普遍存在于水生生态系统中的外源性雌激素，对环境有高度的关注。本研究评估了暴露于 NP 对雄性和雌性斑马鱼（Danio rerio）的运动活性和社会行为的影响，以评估 NP 对鱼类行为的潜在危害，并寻求 NP 污染的潜在生物标志物。鱼被随机分为六组。五组分别接受 0.1、1、10、50 和 100μg/L 名义浓度的 NP，暴露 60 天。第六组对照组给予与其他五组相同的处理，但不添加 NP。检测了运动活性、攻击行为、群体偏好和离开鱼群的行为。NP 暴露对雄性斑马鱼的运动活性有明显影响，而对雌性斑马鱼的运动活性没有明显影响。NP 暴露对雄性和雌性群体的攻击行为和群体偏好都有显著影响。100μg/L NP 明显抑制了雄性和雌雄斑马鱼的运动活性和攻击性，以及雄性和雌雄斑马鱼的群体偏好。在 NP 暴露下，离开鱼群的行为没有明显改变。结果表明，雄性和雌雄斑马鱼的运动活性和攻击性以及雄性和雌雄斑马鱼的群体偏好的变化可以作为 NP 污染的生态相关综合生物标志物。
