Harmsen A G, Stankiewicz M
Trudeau Institute, Inc., Saranac Lake, New York 12983.
J Exp Med. 1990 Sep 1;172(3):937-45. doi: 10.1084/jem.172.3.937.
The importance of CD4+ cells in resistance to Pneumocystis carinii (PC) in PC-susceptible severe combined immunodeficient (SCID) mice that were made resistant to PC by immunocompetent spleen cell transfer, and in conventional PC-resistant mice, was investigated. SCID mice with naturally acquired PC pneumonia (PCP) were given infusions of spleen cells from immunocompetent donors. This reconstitution caused the recipients to resolve their PCP. Treatment of reconstituted SCID mice with anti-CD4 monoclonal antibodies (mAbs) to deplete them of CD4+ cells eliminated their ability to resolve PCP, whereas treating them with anti-CD8 mAb to deplete CD8+ cells had no effect. The findings indicate, therefore, that resistance to PCP in immunologically reconstituted SCID mice is dependent on CD4+ cells. To determine whether CD4+ cells enable conventional mice to resist PCP, B6D2 mice were treated with anti-CD4 mAb to deplete them of CD4+ cells in an attempt to induce PCP. After 10-11 wk of treatment, these mice developed progressive PCP. Taken together, these results indicate that loss of CD4+ cells predisposes mice to PC infection.
研究了CD4 +细胞在易患卡氏肺孢子虫（PC）的严重联合免疫缺陷（SCID）小鼠中对PC的抗性的重要性，这些SCID小鼠通过免疫活性脾细胞转移对PC产生抗性，同时也研究了其在常规抗PC小鼠中的重要性。给患有自然获得性PC肺炎（PCP）的SCID小鼠输注来自免疫活性供体的脾细胞。这种重建使受体小鼠的PCP得到缓解。用抗CD4单克隆抗体（mAb）处理重建的SCID小鼠以耗尽其CD4 +细胞，消除了它们缓解PCP的能力，而用抗CD8 mAb处理以耗尽CD8 +细胞则没有效果。因此，这些发现表明，免疫重建的SCID小鼠对PCP的抗性取决于CD4 +细胞。为了确定CD4 +细胞是否使常规小鼠能够抵抗PCP，用抗CD4 mAb处理B6D2小鼠以耗尽其CD4 +细胞，试图诱导PCP。经过10 - 11周的治疗，这些小鼠发展为进行性PCP。综上所述，这些结果表明CD4 +细胞的缺失使小鼠易患PC感染。
