Roy S, Fitz-Gibbon L, Poulin L, Wainberg M A
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
Immunology. 1988 Jun;64(2):233-9.
We have infected peripheral blood-derived monocyte/macrophage cultures with HIV-1 in order to determine the effect of such infection on cellular immunoregulatory function. We have confirmed that monocytes/macrophages are susceptible to infection by HIV-1, as determined by in situ hybridization using a HIV-1-specific RNA probe and by the presence of reverse transcriptase activity in culture supernatants. The cells employed efficiently supported viral replication in the absence of significant cytopathic effect, and secreted as little as 20% of the amount of IL-1 activity of uninfected controls in response to stimulation with either latex beads or lipopolysaccharide. This effect was not observed when UV-inactivated HIV-1 was used to infect the cells.
我们用HIV-1感染外周血来源的单核细胞/巨噬细胞培养物，以确定这种感染对细胞免疫调节功能的影响。我们已经证实，单核细胞/巨噬细胞易受HIV-1感染，这是通过使用HIV-1特异性RNA探针的原位杂交以及培养上清液中逆转录酶活性的存在来确定的。所使用的细胞在没有明显细胞病变效应的情况下有效地支持病毒复制，并且在用乳胶珠或脂多糖刺激时，分泌的IL-1活性仅为未感染对照的20%。当用紫外线灭活的HIV-1感染细胞时，未观察到这种效应。
