VpreB基因和lambda 5前B细胞特异性基因所编码的蛋白质能够相互结合，并与μ重链结合。
The proteins encoded by the VpreB and lambda 5 pre-B cell-specific genes can associate with each other and with mu heavy chain.
作者信息
Karasuyama H, Kudo A, Melchers F
机构信息
Basel Institute for Immunology, CH-4005, Switzerland.
出版信息
J Exp Med. 1990 Sep 1;172(3):969-72. doi: 10.1084/jem.172.3.969.
Abstract
The murine pre-B cell-specific genes VpreB and lambda 5, as well as the murine gene for mu heavy chain, were introduced into Ltk- fibroblast cells which normally do not express these genes. Stable transfectants carrying these genes produced the corresponding proteins of 15.5, 21.5, and 75 kD. They secreted the three proteins as a triple complex that could be immunoprecipitated by mu heavy chain-specific antibodies, consisting of one VpreB, one lambda 5, and one mu heavy chain. The mu heavy chain and lambda 5 were disulfide-bonded with each other, while the VpreB protein was noncovalently associated. These experiments proved that the VpreB, lambda 5 and mu H chain proteins can form a heavy/light chain-like heterocomplex.
摘要
将鼠源前B细胞特异性基因VpreB和λ5，以及鼠源μ重链基因导入通常不表达这些基因的Ltk-成纤维细胞中。携带这些基因的稳定转染子产生了相应的15.5、21.5和75 kD的蛋白质。它们将这三种蛋白质分泌为一种三聚体复合物，该复合物可被μ重链特异性抗体免疫沉淀，该复合物由一条VpreB、一条λ5和一条μ重链组成。μ重链和λ5通过二硫键相互结合，而VpreB蛋白是非共价结合的。这些实验证明VpreB、λ5和μ重链蛋白可以形成重链/轻链样异源复合物。
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