Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, Germany.
Arch Oral Biol. 2011 Jun;56(6):533-9. doi: 10.1016/j.archoralbio.2010.11.019. Epub 2010 Dec 19.
The purpose of the study was to investigate whether dentine irradiation with a pulsed CO(2) laser (10.6μm) emitting pulses of 10 ms is capable of reducing dentine calcium and phosphorus losses in an artificial caries model.
The 90 dentine slabs obtained from bovine teeth were randomly divided into six groups (n=15): negative control group (GC); positive control group, treated with fluoride 1.23% (GF); and laser groups irradiated with 8 J/cm(2) (L8); irradiated as in L8+fluoride 1.23% (L8F); irradiated with 11 J/cm(2) (L11); irradiated as in L11+fluoride 1.23% (L11F). After laser irradiation the samples were submitted to a pH-cycling model for 9 days. The calcium and phosphorous contents in the de- and remineralization solutions were measured by means of inductively coupled plasma optical emission spectrometer--ICP-OES. Additionally intrapulpal temperature measurements were performed. The obtained data were analysed by means of ANOVA and Tukey's test (α=0.05).
In the demineralization solutions the groups L11F and GF presented significantly lower means of calcium and phosphorous losses than the control group; and in L11F means were significantly lower than in the fluoride group. Both irradiation parameters tested caused intrapulpal temperature increase below 2°C.
It can be concluded that under the conditions of this study, CO(2) laser irradiation (10.6 μm) with 11 J/cm(2) (540 mJ and 10 Hz) of fluoride treated dentine surfaces decreases the loss of calcium and phosphorous in the demineralization process and does not cause excessive temperature increase inside the pulp chamber.
本研究旨在探讨 10.6μm 脉冲 CO₂激光（10ms 脉冲）辐照牙本质是否能减少人工龋模型中牙本质钙、磷的丢失。
从牛牙获得的 90 块牙本质板被随机分为六组（n=15）：阴性对照组（GC）；阳性对照组，用 1.23%氟化物处理（GF）；激光组用 8J/cm²（L8）辐照；与 1.23%氟化物一起辐照（L8F）；用 11J/cm²（L11）辐照；与 1.23%氟化物一起辐照（L11F）。激光照射后，将样品置于 pH 循环模型中 9 天。通过电感耦合等离子体光学发射光谱仪（ICP-OES）测量脱矿和再矿化溶液中的钙和磷含量。此外，还进行了牙髓内温度测量。采用方差分析和 Tukey 检验（α=0.05）对所得数据进行分析。
在脱矿溶液中，L11F 和 GF 组的钙和磷丢失均值明显低于对照组；而 L11F 组的均值明显低于氟化物组。两种测试的辐照参数均导致牙髓内温度升高低于 2°C。
在本研究条件下，11J/cm²（540mJ，10Hz）CO₂激光（10.6μm）辐照氟处理牙本质表面可减少脱矿过程中钙、磷的丢失，且不会导致牙髓腔内温度过度升高。
