Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zürich, Plattenstrasse 11, CH-8032 Zürich, Switzerland.
Arch Oral Biol. 2011 Dec;56(12):1541-7. doi: 10.1016/j.archoralbio.2011.06.010. Epub 2011 Jul 7.
Ninety-six dentine samples were prepared from human premolars and randomly assigned to eight groups (G1-G8). Samples were treated for 30s with the following solutions: placebo (G1/G2), amine fluoride (Elmex fluid; G3/G4), cerium chloride (G5/G6) and combined fluoride/cerium chloride application (G7/G8). Samples of groups G2, G4, G6 and G8 were additionally irradiated with a carbon dioxide laser through the solutions for 30s. Acid resistance was assessed in a six-time 5-min consecutive lactic acid (pH 3.0) erosion model and calcium release was determined by atomic absorption spectroscopy (AAS). Furthermore, six additional samples per group were prepared and subjected to EDS-analysis.
In the non-irradiated groups, specimens of G1 (placebo) showed the highest calcium release when compared to the other treatments (G3, G5 and G7). The highest acid resistance was observed for G7. In G3, calcium release was lower than in G5, but higher than in G7. In general (except for the placebo groups), calcium release in the laser-irradiated groups was higher compared with the respective non-irradiated groups. EDS showed a replacement of calcium by cerium and of phosphor by fluoride.
The highest anti-erosive potential was found after combined cerium chloride and amine fluoride application. Laser irradiation had not adjunctive effect.
从人前磨牙中制备 96 个牙本质样本，并将其随机分配到 8 个组（G1-G8）。用以下溶液处理样本 30s：安慰剂（G1/G2）、胺氟化物（Elmex 液；G3/G4）、氯化铈（G5/G6）和氟/铈联合应用（G7/G8）。G2、G4、G6 和 G8 组的样本还通过溶液额外用二氧化碳激光照射 30s。通过六次连续 5 分钟的乳酸（pH 3.0）侵蚀模型评估耐酸性，并通过原子吸收光谱法（AAS）测定钙释放量。此外，每组另外准备 6 个样本进行 EDS 分析。
在未照射组中，与其他处理（G3、G5 和 G7）相比，G1（安慰剂）组的标本钙释放量最高。G7 组的耐酸性最高。G3 组的钙释放量低于 G5 组，但高于 G7 组。一般来说（安慰剂组除外），激光照射组的钙释放量高于相应的未照射组。EDS 显示钙被铈取代，磷被氟取代。
在联合使用氯化铈和胺氟化物后，发现了最大的抗侵蚀潜力。激光照射没有附加效果。
