Department of Biology, Boston University, Boston, MA 02215, USA.
Development. 2011 Jan;138(2):327-38. doi: 10.1242/dev.057943.
The Bcl-2 family has been shown to regulate mitochondrial dynamics during cell death in mammals and C. elegans, but evidence for this in Drosophila has been elusive. Here, we investigate the regulation of mitochondrial dynamics during germline cell death in the Drosophila melanogaster ovary. We find that mitochondria undergo a series of events during the progression of cell death, with remodeling, cluster formation and uptake of clusters by somatic follicle cells. These mitochondrial dynamics are dependent on caspases, the Bcl-2 family, the mitochondrial fission and fusion machinery, and the autophagy machinery. Furthermore, Bcl-2 family mutants show a striking defect in cell death in the ovary. These data indicate that a mitochondrial pathway is a major mechanism for activation of cell death in Drosophila oogenesis.
Bcl-2 家族已被证明可在哺乳动物和秀丽隐杆线虫的细胞死亡过程中调节线粒体动力学，但在果蝇中这方面的证据一直难以捉摸。在这里，我们研究了果蝇卵巢生殖细胞死亡过程中线粒体动力学的调节。我们发现，在线粒体在细胞死亡过程中的一系列事件中，经历了重塑、聚类形成以及被体细胞滤泡细胞摄取的过程。这些线粒体动力学依赖于半胱天冬酶、Bcl-2 家族、线粒体分裂和融合机制以及自噬机制。此外，Bcl-2 家族突变体在卵巢中的细胞死亡中表现出明显的缺陷。这些数据表明，线粒体途径是果蝇卵子发生中激活细胞死亡的主要机制。
