Bafna Anand, Mishra Shrihari
Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018, India.
Sci Pharm. 2010 Jan-Mar;78(1):21-31. doi: 10.3797/scipharm.0904-16. Epub 2009 Dec 6.
The alkaloidal fraction (AFCP) of roots of Cissampelos pareira Linn. was screened for in-vitro antioxidant activity and immunomodulatory activity in mice. The HPTLC finger print profile was also established for the identification of AFCP which was found to contain 0.176 % of berberine. AFCP possess strong antioxidant activity which was revealed by its ability to scavenge the stable free radical DPPH, superoxide ion and to inhibit lipid peroxidation in rat liver homogenate induced by iron/ADP/Ascorbate complex. AFCP was found to have significant immunosuppressive activity at lower doses (25 and 50 mg/kg) while no activity was observed at higher doses (75 and 100 mg/kg). Humoral antibody titre was significantly (p<0.01) lowered by AFCP at the doses of 25 and 50 mg/kg. Delayed type hypersensitivity response was also significantly (p<0.01) suppressed by the AFCP at the dose of 75 mg/kg. Thus the present study revealed the immunosuppressive and antioxidant activities of the alkaloidal fraction of C. pareira roots.
对锡生藤（Cissampelos pareira Linn.）根的生物碱部分（AFCP）进行了体外抗氧化活性和对小鼠免疫调节活性的筛选。还建立了HPTLC指纹图谱以鉴定AFCP，发现其含有0.176%的小檗碱。AFCP具有很强的抗氧化活性，这通过其清除稳定自由基DPPH、超氧离子以及抑制铁/ADP/抗坏血酸复合物诱导的大鼠肝匀浆脂质过氧化的能力得以体现。发现AFCP在较低剂量（25和50 mg/kg）时有显著的免疫抑制活性，而在较高剂量（75和100 mg/kg）时未观察到活性。AFCP在25和50 mg/kg剂量时显著（p<0.01）降低了体液抗体滴度。AFCP在75 mg/kg剂量时也显著（p<0.01）抑制了迟发型超敏反应。因此，本研究揭示了锡生藤根生物碱部分的免疫抑制和抗氧化活性。
