Cao Z F
Department of Physiology, Hunan Medical University, Changsha, China.
Planta Med. 1996 Oct;62(5):413-4. doi: 10.1055/s-2006-957928.
The scavenging effects of tetrandrine (TD) on active oxygen radicals were investigated through chemiluminescence experiments and oxidation of ferrocytochrome c. The superoxide anions (O2-.) were generated either in hypoxanthine/xanthine oxidase (HX-XOD) system or by means of auto-oxidation of erythrocytes. It was found that TD (7.5 microM) could remarkably scavenge O2-. in the HX-XOD system and that the effect was concentration-dependent. The scavenging effect of TD on O2-. generated from auto-oxidation in erythrocytes was not as strong as in the HX-XOD system. The hydroxyl radicals (.OH) were generated in aqueous solution of H2O2 irradiated by UV, it was also found that TD (15 microM) effectively scavenged .OH in that system. This study led to the conclusion that the antioxidant property of TD may account for some of its pharmacological effects.
通过化学发光实验和亚铁细胞色素c的氧化，研究了粉防己碱（TD）对活性氧自由基的清除作用。超氧阴离子（O2-.）可在次黄嘌呤/黄嘌呤氧化酶（HX-XOD）系统中产生，也可通过红细胞的自动氧化产生。结果发现，TD（7.5微摩尔）能在HX-XOD系统中显著清除O2-.，且该作用具有浓度依赖性。TD对红细胞自动氧化产生的O2-.的清除作用不如在HX-XOD系统中强。羟基自由基（.OH）在紫外线照射的H2O2水溶液中产生，还发现TD（15微摩尔）能在该系统中有效清除.OH。本研究得出结论，TD的抗氧化特性可能是其某些药理作用的原因。
