青霉素和头孢菌素生物合成的首个中间体δ-（L-α-氨基己二酰基）-L-半胱氨酸的无细胞合成。
Cell-free synthesis of delta-(L-alpha-aminoadipyl)-L-cysteine, the first intermediate of penicillin and cephalosporin biosynthesis.
作者信息
Banko G, Wolfe S, Demain A L
出版信息
Biochem Biophys Res Commun. 1986 May 29;137(1):528-35. doi: 10.1016/0006-291x(86)91242-8.
PMID:3718519
Abstract
delta-(L-alpha-aminoadipyl)-L-cysteine synthetase (LL-AC synthetase) activity has been found in extracts of Cephalosporium acremonium C-10. The enzyme extract carries out a linear synthesis of LL-AC from its constituent amino acids for at least 6 hours. The reaction is dependent on active enzyme, time, L-alpha-aminoadipate, L-cysteine, ATP and Mn2+ or Mg2+. The activity is stabilized by glycerol.
摘要
在顶头孢霉C-10提取物中发现了δ-（L-α-氨基己二酰基）-L-半胱氨酸合成酶（LL-AC合成酶）活性。该酶提取物可从其组成氨基酸进行LL-AC的线性合成，至少持续6小时。该反应依赖于活性酶、时间、L-α-氨基己二酸、L-半胱氨酸、ATP以及Mn2+或Mg2+。甘油可使该活性稳定。
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