Biochemical Sciences Division, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune, 411008, India.
J Appl Genet. 2011 Feb;52(1):9-21. doi: 10.1007/s13353-010-0016-y. Epub 2010 Dec 23.
Chickpea is one of the most important leguminous cool season food crops, cultivated prevalently in South Asia and Middle East. The main objective of this study was to identify quantitative trait loci (QTLs) associated with seven agronomic and yield traits in two recombinant inbred line populations of chickpea derived from the crosses JG62 × Vijay (JV population) and Vijay × ICC4958 (VI population) from at least three environments. Single locus QTL analysis involved composite interval mapping (CIM) for individual traits and multiple-trait composite interval mapping (MCIM) for correlated traits to detect pleiotropic QTLs. Two-locus analysis was conducted to identify the main effect QTLs (M-QTLs), epistatic QTLs (E-QTLs) and QTL × environment interactions. Through CIM analysis, a total of 106 significant QTLs (41 in JV and 65 in VI populations) were identified for the seven traits, of which one QTL each for plant height and days to maturity was common in both the populations. Six pleiotropic QTLs that were consistent over the environments were also identified. LG2 in JV and LG1a in VI contained at least one QTL for each trait. Hence, concentrating on these LGs in molecular breeding programs is most likely to bring simultaneous improvement in these traits.
鹰嘴豆是一种最重要的豆科冷季粮食作物，主要种植在南亚和中东地区。本研究的主要目的是在两个由 JG62×Vijay（JV 群体）和 Vijay×ICC4958（VI 群体）杂交衍生的重组自交系群体中，至少在三个环境下，鉴定与 7 个农艺和产量性状相关的数量性状位点（QTLs）。单座标 QTL 分析涉及单个性状的复合区间作图（CIM）和相关性状的多性状复合区间作图（MCIM），以检测多效 QTLs。双座标分析用于鉴定主效 QTLs（M-QTLs）、上位性 QTLs（E-QTLs）和 QTL×环境互作。通过 CIM 分析，共鉴定到 7 个性状的 106 个显著 QTLs（JV 群体 41 个，VI 群体 65 个），其中株高和成熟天数各有 1 个 QTL 在两个群体中是共有的。还鉴定到 6 个在不同环境下一致的多效 QTLs。JV 中的 LG2 和 VI 中的 LG1a 每个性状至少包含 1 个 QTL。因此，在分子育种计划中集中于这些 LGs 很可能会同时改善这些性状。
