Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
Department of Genetics, Osmania University, Hyderabad, India.
PLoS One. 2021 May 14;16(5):e0251669. doi: 10.1371/journal.pone.0251669. eCollection 2021.
Unravelling the genetic architecture underlying yield components and agronomic traits is important for enhancing crop productivity. Here, a recombinant inbred line (RIL) population, developed from ICC 4958 and DCP 92-3 cross, was used for constructing linkage map and QTL mapping analysis. The RIL population was genotyped using a high-throughput Axiom®CicerSNP array, which enabled the development of a high-density genetic map consisting of 3,818 SNP markers and spanning a distance of 1064.14 cM. Analysis of phenotyping data for yield, yield components and agronomic traits measured across three years together with genetic mapping data led to the identification of 10 major-effect QTLs and six minor-effect QTLs explaining up to 59.70% phenotypic variance. The major-effect QTLs identified for 100-seed weight, and plant height possessed key genes, such as C3HC4 RING finger protein, pentatricopeptide repeat (PPR) protein, sugar transporter, leucine zipper protein and NADH dehydrogenase, amongst others. The gene ontology studies highlighted the role of these genes in regulating seed weight and plant height in crop plants. The identified genomic regions for yield, yield components, and agronomic traits, and the closely linked markers will help advance genetics research and breeding programs in chickpea.
解析产量构成和农艺性状的遗传结构对于提高作物生产力至关重要。本研究利用 ICC 4958 和 DCP 92-3 杂交衍生的重组自交系（RIL）群体，构建连锁图谱和 QTL 作图分析。该 RIL 群体通过高通量 Axiom®CicerSNP 芯片进行基因型分析，成功开发了一张高密度遗传图谱，包含 3818 个 SNP 标记，覆盖 1064.14cM 的距离。对三年间测定的产量、产量构成和农艺性状的表型数据以及遗传图谱数据进行分析，共鉴定到 10 个主效 QTL 和 6 个微效 QTL，可解释高达 59.70%的表型变异。鉴定到的与百粒重和株高相关的主效 QTL 具有 C3HC4 RING 指蛋白、五肽重复（PPR）蛋白、糖转运蛋白、亮氨酸拉链蛋白和 NADH 脱氢酶等关键基因。基因本体论研究强调了这些基因在调控作物种子重量和株高方面的作用。鉴定到的与产量、产量构成和农艺性状相关的基因组区域及其紧密连锁的标记将有助于推进鹰嘴豆的遗传研究和育种计划。
