da Silva Fabiano Fernandes, de Souza Renato Aparecido, Pacheco Marcos Tadeu Tavares, Ribeiro Wellington, da Silva Marcos Augusto Souza Rodrigues, Miranda Humberto, Salgado Miguel Angel Castillo, de Melo Castilho Julio Cezar, Silveira Landulfo
Grupo de Estudo e Pesquisa em Ciência da Saúde, Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas-Campus Muzambinho, Bairro Morro Preto, Muzambinho, MG, Brazil.
Photomed Laser Surg. 2011 Apr;29(4):217-25. doi: 10.1089/pho.2010.2784. Epub 2010 Dec 23.
Raman spectroscopy was employed to evaluate the effect of different swimming intensities on femoral bone composition in an animal model.
Intense swimming exercise may affect bone mineralization, and Raman technique has been shown to be effective in evaluating tissue composition (phosphate minerals and carbonate apatites - bands at 960 and 1170 cm(-1), as well as collagen matrix - amide I band at 1660 cm(-1)).
Eighteen female Swiss Webster mice were separated into three groups (n = 6 per group) of sedentary (SED), and swimming with an intensity of 40% (PT-40) and 80% (PT-80) of the maximum load, with 6 weeks of training. Near-infrared Raman spectra (830 nm wavelength and 80 mW laser power) were obtained with a dispersive Raman spectrometer using a CCD camera and imaging spectrograph with 30-s integration time. Spectra were collected in the medial and lateral diaphysis of the femur and principal components analysis (PCA) was employed to extract features of the Raman bands of bone and to perform quantitative analysis.
PC1 vector resembles Raman spectra and carries information about apatite minerals and some contribution from organic matrix. A statistically significant difference was found in the PC1 scores (ANOVA, p < 0.05), indicating lower mineral concentrations in the femur in both the PT-40 and PT-80 groups compared to the SED group. These results corroborated with the radiographic assessment of bone density.
Raman technique associated with PCA statistics showed that intense swimming exercise may affect bone mineralization and remodeling in a mouse model of training.
采用拉曼光谱法评估动物模型中不同游泳强度对股骨骨成分的影响。
高强度游泳运动可能会影响骨矿化，且拉曼技术已被证明在评估组织成分（磷酸盐矿物质和碳酸盐磷灰石——960和1170 cm⁻¹处的谱带，以及胶原基质——1660 cm⁻¹处的酰胺I谱带）方面是有效的。
将18只雌性瑞士韦伯斯特小鼠分为三组（每组n = 6），分别为久坐组（SED）、以最大负荷40%强度游泳组（PT - 40）和80%强度游泳组（PT - 80），进行为期6周的训练。使用配备电荷耦合器件相机和成像光谱仪的色散拉曼光谱仪，在830 nm波长和80 mW激光功率下，以30秒积分时间获取近红外拉曼光谱。在股骨的内侧和外侧骨干收集光谱，并采用主成分分析（PCA）提取骨拉曼谱带的特征并进行定量分析。
PC1向量类似于拉曼光谱，并携带有关磷灰石矿物质的信息以及有机基质的一些贡献。在PC1得分上发现了统计学上的显著差异（方差分析，p < 0.05），表明与SED组相比，PT - 40组和PT - 80组的股骨矿物质浓度较低。这些结果与骨密度的影像学评估结果一致。
与PCA统计相关的拉曼技术表明，高强度游泳运动可能会影响小鼠训练模型中的骨矿化和重塑。
