Ayats Josefina, Martín-Mazuelos Estrella, Pemán Javier, Quindós Guillermo, Sánchez Fernando, García-Rodríguez Julio, Guarro Josep, Guinea Jesús, Linares María J, Pontón José, Rodríguez-Tudela Juan L, Cuenca-Estrella Manuel
Servicio de Microbiología, Hospital Universitario de Bellvitge, L'Hospitalet de Llobregat, Barcelona, España.
Enferm Infecc Microbiol Clin. 2011 Jan;29(1):39.e1-15. doi: 10.1016/j.eimc.2010.08.005. Epub 2010 Dec 22.
These guidelines are an update of recommendations for the diagnosis of invasive fungal infections by the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC) published in 2004 (Enferm Infecc Microbiol Clin. 2004, 22:32-9). In this updated version of the guidelines, a comprehensive review of the most recent diagnostic innovations and levels of evidence to recommend those diagnostic procedures are included. We first analyse conventional diagnostic methods, microscopic examination and culture, underlining their limitations which have led to the development of alternative methods, such as fungal antigen and DNA quantification. Those alternative methods of diagnosis are analysed by fungal infection. We also briefly review the methods for molecular identification of fungal species and recommendations for carrying out susceptibility tests for antifungal drugs, including reference procedures, commercial techniques and their indications.
本指南是西班牙临床微生物学和传染病学会（SEIMC）于2004年发布的侵袭性真菌感染诊断建议的更新版（《Enferm Infecc Microbiol Clin.》2004年，第22卷：32 - 9页）。在本指南的更新版本中，纳入了对最新诊断创新以及推荐这些诊断程序的证据水平的全面综述。我们首先分析传统诊断方法，即显微镜检查和培养，强调其局限性，这些局限性促使了替代方法的发展，如真菌抗原和DNA定量分析。按真菌感染类型分析那些替代诊断方法。我们还简要回顾了真菌菌种分子鉴定方法以及抗真菌药物药敏试验的建议，包括参考程序、商业技术及其适用情况。
