Kobayashi N, McEntee K
Laboratory of Biomedical and Environmental Sciences, University of California, Los Angeles 90024.
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6550-4. doi: 10.1073/pnas.87.17.6550.
Transcription of the DNA damage-responsive gene, DDRA2, of Saccharomyces cerevisiae is activated by heat shock treatment as well as by mutagen/carcinogen exposure. Deletion analysis of upstream noncoding sequence indicated that sequences between approximately -190 and -140 base pairs were necessary for heat shock and DNA damage regulation of transcription. Fusion of this region to a CYC1-lacZ reporter gene demonstrated that the sequence between -202 and -165 base pairs was sufficient for basal level and heat shock-induced expression. This DNA sequence was unable to bind heat shock transcription factor as judged by binding competition experiments in vitro. These results indicate that yeast possesses a second, heat shock transcription factor-independent mechanism for activating transcription in response to thermal stress.
酿酒酵母的DNA损伤反应基因DDRA2的转录可通过热休克处理以及诱变剂/致癌物暴露来激活。对上游非编码序列的缺失分析表明，大约-190至-140碱基对之间的序列对于转录的热休克和DNA损伤调节是必需的。将该区域与CYC1-lacZ报告基因融合表明，-202至-165碱基对之间的序列足以实现基础水平和热休克诱导的表达。通过体外结合竞争实验判断，该DNA序列无法结合热休克转录因子。这些结果表明，酵母拥有第二种独立于热休克转录因子的机制，用于在热应激反应中激活转录。
