Cisak Ewa, Wójcik-Fatla Angelina, Zając Violetta, Sroka Jacek, Buczek Alicja, Dutkiewicz Jacek
Unit of Zoonotic Diseases, Institute of Agricultural Medicine, Lublin, Poland.
Ann Agric Environ Med. 2010;17(2):283-6.
A total of 119 unpasteurized milk samples taken from 63 cows, 29 goats and 27 sheep bred on 8 farms situated on the territory of the Lublin province (eastern Poland), an area of risk of tick-borne encephalitis (TBE), were examined for the presence of RNA of tick-borne encephalitis virus (TBEV) by the nested RT-PCR method. Milk samples were also tested for the presence of anti-TBEV antibodies by ELISA test. By RT-PCR, the greatest prevalence of TBE virus was found in the milk of sheep (22.2%), followed by milk of goats (20.7%) and cows (11.1%). By ELISA, the greatest prevalence of anti- TBEV antibodies was found also in the milk of sheep (14.8%), followed by milk of cows (3.2%) and goats (0%). The results suggest a potential risk of infection with TBEV by drinking raw milk on endemic areas of TBE, and indicate a need for milk pasteurization before consumption.
从位于波兰东部卢布林省（蜱传脑炎风险地区）8个农场饲养的63头奶牛、29只山羊和27只绵羊采集了共计119份未经巴氏消毒的牛奶样本，采用巢式RT-PCR方法检测蜱传脑炎病毒（TBEV）的RNA。还通过ELISA试验检测牛奶样本中抗TBEV抗体的存在情况。通过RT-PCR发现，绵羊奶中TBE病毒的流行率最高（22.2%），其次是山羊奶（20.7%）和牛奶（11.1%）。通过ELISA检测，抗TBEV抗体的流行率最高同样出现在绵羊奶中（14.8%），其次是牛奶（3.2%）和山羊奶（0%）。结果表明，在蜱传脑炎流行地区饮用生牛奶存在感染TBEV的潜在风险，并表明在食用前需要对牛奶进行巴氏消毒。
